Antibody-drug Conjugate Information
General Information of This Antibody-drug Conjugate (ADC)
| ADC ID |
DRG0RPGUJ
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| ADC Name |
Enfortumab-MMAE
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| Synonyms |
Enfortumab-MMAE
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| Organization |
Kyushu University.
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| Drug Status |
Investigative
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| Antibody Name |
Enfortumab
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Antibody Info | ||||
| Antigen Name |
Nectin-4 (NECTIN4)
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Antigen Info | ||||
| Payload Name |
Monomethyl auristatin E
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Payload Info | ||||
| Therapeutic Target |
Microtubule (MT)
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Target Info | ||||
| Linker Name |
Undisclosed
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| Conjugate Type |
Random conjugation through reduced inter-chain cysteines.
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General Information of The Activity Data Related to This ADC
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
7.81۬.31 nM
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| Method Description |
Cells were seeded into 96-well plates (5000 cells/well) and incubated for 24 h at 37°C in 5% CO2. To investigate the effect of NECTIN4-targeted ADC, cells were incubated with medium containing enfortumab or enfortumab-MMAE conjugate. After 72 h of incubation, 10 uL of CCK-8 solution was added to each well and incubated for 3 h at 37°C. Then, absorbance at 450 nm was measured using a microplate reader (Bio-Rad Laboratories Inc.). The IC50 of the NECTIN4-targeted ADC was calculated using GraphPad Prism 7 software (GraphPad Software, San Diego, CA, USA).
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| In Vitro Model | Extramammary Paget disease | KS-EMPD-1 cells | CVCL_C4LP | ||
References