Antibody-drug Conjugate Information
General Information of This Antibody-drug Conjugate (ADC)
| ADC ID |
DRG0HXUJC
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| ADC Name |
37520726 I1-DXd
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| Synonyms |
I1-DXd
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| Organization |
University of Chinese Academy of Sciences; Zhejiang Cancer Hospital; Chinese Academy of Sciences; Nagoya City University; MabPlex International; Tianjin University; Tongji University; Boston Children's Hospital; Harvard Medical School.
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| Drug Status |
Investigative
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| Structure |
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| Antibody Name |
undisclosed
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| Antigen Name |
Intercellular adhesion molecule 1 (ICAM1)
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Antigen Info | ||||
| Payload Name |
DX-8951 derivative (DXd)
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Payload Info | ||||
| Therapeutic Target |
DNA topoisomerase 1 (TOP1)
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Target Info | ||||
| Linker Name |
Mc-Gly-Gly-Phe-Gly
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Linker Info | ||||
| Conjugate Type |
Random conjugation through reduced inter-chain cysteine.
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| Combination Type |
Deruxtecan
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General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
2.48۪.88 nM
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| Method Description |
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.
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| In Vitro Model | Differentiated thyroid carcinoma, Thyroid gland papillary carcinoma | IHH4 cells | CVCL_2960 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
54.1䔷.4 nM
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| Method Description |
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.
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| In Vitro Model | Thyroid carcinoma | BCPAP cells | CVCL_0153 | ||
References