Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0ZLPTS
ADC Name
37520726 I1-MMAE
Synonyms
I1-MMAE
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Organization
University of Chinese Academy of Sciences; Zhejiang Cancer Hospital; Chinese Academy of Sciences; Nagoya City University; MabPlex International; Tianjin University; Tongji University; Boston Children's Hospital; Harvard Medical School.
Drug Status
Investigative
Structure
Antibody Name
undisclosed
Antigen Name
Intercellular adhesion molecule 1 (ICAM1)
  Antigen Info 
Payload Name
Monomethyl auristatin E
  Payload Info 
Therapeutic Target
Microtubule (MT)
  Target Info 
Linker Name
Mc-Val-Cit-PABC
  Linker Info 
Combination Type
Vedotin
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 4 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal inhibitory Concentration (lC50) 
1.69&#1770.31
nM
CVCL_2960
Differentiated thyroid carcinoma, Thyroid gland papillary carcinoma
Half Maximal inhibitory Concentration (lC50) 
7.35&#1771.45
nM
CVCL_1054
Thyroid gland anaplastic carcinoma
Half Maximal inhibitory Concentration (lC50) 
15.59&#1774.18
nM
CVCL_M839
Thyroid gland anaplastic carcinoma, Anaplastic thyroid carcinoma
Half Maximal inhibitory Concentration (lC50) 
68.6&#17711.2
nM
CVCL_0153
Thyroid carcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 4 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50)
1.69&#1770.31 nM
Method Description
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.

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In Vitro Model Differentiated thyroid carcinoma, Thyroid gland papillary carcinoma IHH4 cells CVCL_2960
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50)
7.35&#1771.45 nM
Method Description
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.

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In Vitro Model Thyroid gland anaplastic carcinoma 8505C cells CVCL_1054
Experiment 3 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50)
15.59&#1774.18 nM
Method Description
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.

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In Vitro Model Thyroid gland anaplastic carcinoma, Anaplastic thyroid carcinoma TCO1 cells CVCL_M839
Experiment 4 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50)
68.6&#17711.2 nM
Method Description
Human thyroid cancer cells were seeded in a 96-well plate at a density of 3000 cells per well overnight. The cell culture medium was replaced with the medium containing either chemo drugs (maximum concentration: 117.11 umol/L) or ICAM1-ADCs at serial diluted concentrations (maximum concentration: 0.67 umol/L). After 96h, cell cytotoxicity was determined by using a CCK-8 kit (KeyGEN Biotech, China) following the manufacturer's protocol. The absorbance at 450 nm was measured with an ELISA browser (Bio-Tek EL 800, USA). The experiments were repeated three times.

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In Vitro Model Thyroid carcinoma BCPAP cells CVCL_0153
References
Ref 1 ICAM1 antibody drug conjugates exert potent antitumor activity in papillary and anaplastic thyroid carcinoma