Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)

ADC ID	DRG0ZGUAA
ADC Name	B4-SMCC-DC4
Synonyms	B4-SMCC DC4 Click to Show/Hide
Drug Status	Investigative
Indication	In total 2 Indication(s) Acute promyelocytic leukemia [ICD11:2A60] Investigative Burkitt lymphoma [ICD11:2A85] Investigative
Drug-to-Antibody Ratio	3.5-5
Structure
	3D MOL	2D MOL
Antibody Name	Anti-CD19 mAb B4		Antibody Info
Antigen Name	B-lymphocyte antigen CD19 (CD19)		Antigen Info
Payload Name	Phosphate prodrugs 40 (DC4)		Payload Info
Therapeutic Target	Human Deoxyribonucleic acid (hDNA)		Target Info
Linker Name	Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC)		Linker Info
Conjugate Type	Lysine residue on the antibody is conjugated via an amide bond to the linker-payload moiety.
Combination Type	SMCC-DC4

General Information of The Activity Data Related to This ADC

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Standard Type	Value	Units	Cell Line	Disease Model
Half Maximal Inhibitory Concentration (IC50)	1.2	nM	Ramos cells	Burkitt lymphoma
Half Maximal Inhibitory Concentration (IC50)	2.6	nM	HL-60 cells	Adult acute myeloid leukemia

Full List of Activity Data of This Antibody-drug Conjugate

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	1.20 nM	Positive CD19 expression (CD19+++/++)
Method Description	Cytotoxicity of B4-SPP-DCx Conjugates (with acid phosphatase treatment) against Ramos (Ag+) and HL60/s (Ag-) Cells.
In Vitro Model	Burkitt lymphoma	Ramos cells		CVCL_0597
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	2.60 nM	Negative CD19 expression(CD19-)
Method Description	Cytotoxicity of B4-SPP-DCx Conjugates (with acid phosphatase treatment) against Ramos (Ag+) and HL60/s (Ag-) Cells.
In Vitro Model	Adult acute myeloid leukemia	HL-60 cells		CVCL_0002

References

Ref 1	Synthesis and biological evaluation of antibody conjugates of phosphate prodrugs of cytotoxic DNA alkylators for the targeted treatment of cancer. J Med Chem. 2012 Jan 26;55(2):766-82. doi: 10.1021/jm201284m.