Payload Information

General Information of This Payload

Payload ID	PAY0JOHRS
Name	Diphtheria toxin containing streptococcal protein G (DT3C)
Synonyms	Diphtheria toxin containing streptococcal protein G (DT3C) Click to Show/Hide
Target(s)	Eukaryotic elongation factor 2 (EEF2)	Target Info

The activity data of This Payload

Standard Type	Value	Units	Cell line	Disease Model	Cell line ID	Reference
Survival Rate	≈96.3	%	PC-9 cells	Lung adenocarcinoma	CVCL_B260	[1]

Each Antibody-drug Conjugate Related to This Payload

Full Information of The Activity Data of The ADC(s) Related to This Payload

H8R23-DT3C [Investigative]

ADC Info

Discovered Using Cell Line-derived Xenograft Model

Click To Hide/Show 1 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[2]
Efficacy Data	Tumor Growth Inhibition value (TGI)	≈ 85.90% (Day 4)	High ANTXR2 expression (ANTXR2+++)
Method Description	The H8R23 mAb, or control IgG, and DT3C were incubated at room temperature for 30 min to generate a mAb-DT3C conjugate. Each conjugate theoretically consists of one mAb molecule (150 kDa) and two DT3Cs (140 kDa). Hs-746 T cells were seeded and incubated in the presence of the H8R23-DT3C conjugates or control IgG-DT3C conjugates (03 ug/mL each) for 96 h, then their viability and level of apoptosis were determined as described above. Click to Show/Hide
In Vitro Model	Gastric adenocarcinoma	Hs 746.T cells		CVCL_0333

HSL156 mAb-DT3C conjugate [Investigative]

ADC Info

Revealed Based on the Cell Line Data

Click To Hide/Show 3 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[3]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	1.90 ng/mL	Positive JAM3 expression (JAM3 +++/++)
Method Description	In vitro cytotoxicity of ADCs against a panel of four multiple human small cell cancer cell lines.
In Vitro Model	Lung small cell carcinoma	LU-134-A cells		CVCL_1387
Experiment 2 Reporting the Activity Date of This ADC					[3]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	15.00 ng/mL	Positive JAM3 expression (JAM3 +++/++)
Method Description	In vitro cytotoxicity of ADCs against a panel of four multiple human small cell cancer cell lines.
In Vitro Model	Lung small cell carcinoma	LU-135 cells		CVCL_1389
Experiment 3 Reporting the Activity Date of This ADC					[3]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	15.00 ng/mL	Positive JAM3 expression (JAM3 +++/++)
Method Description	In vitro cytotoxicity of ADCs against a panel of four multiple human small cell cancer cell lines.
In Vitro Model	Lung small cell carcinoma	SBC-5 cells		CVCL_1679

References

Ref 1	Development of a sensitive screening method for selecting monoclonal antibodies to be internalized by cells. Biochem Biophys Res Commun. 2014 Nov 28;454(4):600-3.
Ref 2	Anthrax toxin receptor 2 is a potential therapeutic target for non-small cell lung carcinoma with MET exon 14 skipping mutations. Exp Cell Res. 2022 Apr 15;413(2):113078.
Ref 3	Junctional adhesion molecule 3 is a potential therapeutic target for small cell lung carcinoma. Exp Cell Res. 2023 May 15;426(2):113570. doi: 10.1016/j.yexcr.2023.113570. Epub 2023 Mar 27.