Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0VDJXT |
|||||
|---|---|---|---|---|---|---|
| Antibody Name | MUC1 3D1 |
|||||
| Organization | Dana-Farber Cancer Institute.; National Hospital Organization Tokyo Medical Center.; Keio University. |
|||||
| Synonyms |
MUC1 3D1
Click to Show/Hide
|
|||||
| Antibody Type | Monoclonal antibody (mAb) |
|||||
| Antibody Subtype | Humanized lgG |
|||||
| Antigen Name | Mucin-1 (MUC1) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
40287441 3D1 MMAE [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
1.8 nM
|
Positive MUC1-C expression (MUC1-C+++/++) | ||
| Method Description |
Cells were seeded at a density of 1500-3500 cells per well in 96-well plates. The next day, the cells were treated with different concentrations of the drug. Cell viability and proliferation were assessed using the Alamar Blue Reagent (cat# DAL1100, Thermo Fisher Scientific) following the company protocol. The IC50 values were determined by nonlinear regression of the dose-response data using Prism 10.0 (SCR_002798, GraphPad Software). Fluorescence intensity (560 nm excitation/590 nm emission) was measured in at least triplicate wells.
Click to Show/Hide
|
||||
| In Vitro Model | Invasive breast carcinoma of no special type | MCF-7 cells (ER (D538G)) | CVCL_0031 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
6.3 nM
|
Positive MUC1-C expression (MUC1-C+++/++) | ||
| Method Description |
Cells were seeded at a density of 1500-3500 cells per well in 96-well plates. The next day, the cells were treated with different concentrations of the drug. Cell viability and proliferation were assessed using the Alamar Blue Reagent (cat# DAL1100, Thermo Fisher Scientific) following the company protocol. The IC50 values were determined by nonlinear regression of the dose-response data using Prism 10.0 (SCR_002798, GraphPad Software). Fluorescence intensity (560 nm excitation/590 nm emission) was measured in at least triplicate wells.
Click to Show/Hide
|
||||
| In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
27 nM
|
Positive MUC1-C expression (MUC1-C+++/++) | ||
| Method Description |
Cells were seeded at a density of 1500-3500 cells per well in 96-well plates. The next day, the cells were treated with different concentrations of the drug. Cell viability and proliferation were assessed using the Alamar Blue Reagent (cat# DAL1100, Thermo Fisher Scientific) following the company protocol. The IC50 values were determined by nonlinear regression of the dose-response data using Prism 10.0 (SCR_002798, GraphPad Software). Fluorescence intensity (560 nm excitation/590 nm emission) was measured in at least triplicate wells.
Click to Show/Hide
|
||||
| In Vitro Model | Invasive breast carcinoma of no special type | MCF-7 cells (ER (Y537S)) | CVCL_0031 | ||
References