Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0INRDN |
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| Antibody Name | huNb26/Nb26-Nbh |
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| Organization | Shanghai University of Medicine & Health Sciences Affiliated Zhoupu Hospital. |
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| Synonyms |
huNb26/Nb26-Nbh
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| Antibody Type | Nanobody (Nb) |
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| Antigen Name | Nectin-4 (NECTIN4) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
huNb26/Nb26-Nbh-MMAE [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
44.63 nM
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Low NECTIN4 expression (NECTIN4+) | ||
| Method Description |
Cells in the exponential growth phase were harvested and seeded at a density of 4 × 103 cells per well into 96-well plates, followed by overnight incubation. The 96-well plate was filled with gradient dilutions of the NDC along with complete growth medium. Cells were incubated at 37 °C for 96 h. 20 uL of cell counting Kit-8 (Dojindo) was administered to the cells, which were then incubated at 37 °C for 4 h. After shaking the 96-well plates, absorbance was measured at 450 nm using an enzyme marker. Inhibition % = (1 - absorbance of experimental group) / average absorbance of blank group × 100. IC50 values were ascertained by non-linear regression analysis utilizing GraphPad Prism version 8.0.
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| In Vitro Model | Gastric tubular adenocarcinoma | NCI-N87 cells | CVCL_1603 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
1.04 nM
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Low NECTIN4 expression (NECTIN4+) | ||
| Method Description |
Cells in the exponential growth phase were harvested and seeded at a density of 4 × 103 cells per well into 96-well plates, followed by overnight incubation. The 96-well plate was filled with gradient dilutions of the NDC along with complete growth medium. Cells were incubated at 37 °C for 96 h. 20 uL of cell counting Kit-8 (Dojindo) was administered to the cells, which were then incubated at 37 °C for 4 h. After shaking the 96-well plates, absorbance was measured at 450 nm using an enzyme marker. Inhibition % = (1 - absorbance of experimental group) / average absorbance of blank group × 100. IC50 values were ascertained by non-linear regression analysis utilizing GraphPad Prism version 8.0.
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| In Vitro Model | Gastric tubular adenocarcinoma | NCI-N87 cells | CVCL_1603 | ||
References