Antibody-drug Conjugate Information
General Information of This Antibody-drug Conjugate (ADC)
| ADC ID |
DRG0ZUUMS
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| ADC Name |
TE-1146
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| Synonyms |
TE-1146
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| Organization |
Immunwork, Inc.; Institute of Biomedical Sciences.
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| Drug Status |
Investigative
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| Drug-to-Antibody Ratio |
6
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| Antibody Name |
alpha-CD38 mAb
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Antibody Info | ||||
| Antigen Name |
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38)
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Antigen Info | ||||
| Payload Name |
Lenalidomide
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Payload Info | ||||
| Linker Name |
Undisclosed
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| Conjugate Type |
Random conjugation through reduced inter-chain cysteines.
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General Information of The ADMET Data Related to This ADC(2027 Update)
Excretion
| Standard Type | Value | Units | Description | Reference |
|---|---|---|---|---|
| Elimination Half-Life (t1/2) | 6-7 | day |
We assessed the stability of TE-1146 compared to that of daratumumab or unconjugated alpha-CD38. All three molecules were dissolved in 90% human plasma, incubated at 37 °C for 28 days, and analyzed by ELISA. Daratumumab, alpha-CD38 mAb, and TE-1146 exhibited similar half-lives (T1/2) of 6-7 days in human plasma over the 28 days.
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[1] |
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 0.45 uM | High CD38 expression (CD38 +++) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
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| In Vitro Model | Multiple myeloma, Plasma cell myeloma | H929 cells | CVCL_1600 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 0.45 uM | High CD38 expression (CD38 +++) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
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| In Vitro Model | Plasma cell myeloma, Multiple myeloma | MM.1S cells | CVCL_8792 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | > 10000 uM | Negative CD38 expression (CD38-) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
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| In Vitro Model | Multiple myeloma, Plasma cell myeloma | U266 (CD38-) cells | Homo sapiens | ||