Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0TLAQS |
|||||
|---|---|---|---|---|---|---|
| Antibody Name | alpha-CD38 mAb |
|||||
| Synonyms |
alpha-CD38 mAb
Click to Show/Hide
|
|||||
| Antigen Name | ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (CD38) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
TE-1146 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
0.45 uM
|
High CD38 expression (CD38 +++) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
Click to Show/Hide
|
||||
| In Vitro Model | Multiple myeloma, Plasma cell myeloma | H929 cells | CVCL_1600 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
0.45 uM
|
High CD38 expression (CD38 +++) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
Click to Show/Hide
|
||||
| In Vitro Model | Plasma cell myeloma, Multiple myeloma | MM.1S cells | CVCL_8792 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | > 10000 uM | Negative CD38 expression (CD38-) | ||
| Method Description |
To evaluate the cytotoxic effects of various drugs, H929, U266-CD38-, MM.1S, and Daudi cells were seeded at 5000 cells per well in 96-well plates and cocultured with fresh medium containing different drug concentrations for varying durations (5 h, 1 day, 3 or 5 days) at 37 °C. Cell viability was determined using the alamarBlue cell viability reagent (Thermo Fisher Scientific). About 10 uL alamarBlue cell viability reagent was added to achieve a final concentration of 10% v/v and incubated at 37 °C for 1.5 h. The fluorescence was measured on a microplate reader in arbitrary fluorescent units following excitation at 560 nm and emission at 590 nm.
Click to Show/Hide
|
||||
| In Vitro Model | Multiple myeloma, Plasma cell myeloma | U266 (CD38-) cells | Homo sapiens | ||