Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0VRCOP
ADC Name
CN117337196A Compound89
Synonyms
30kmPEG (SCAPDL1xSCACD47)-3 (Val-Cit-PAB-DEA-SN38)
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Organization
Shenzhen Enduring Biotech Ltd.
Drug Status
Investigative
Drug-to-Antibody Ratio
1
Structure
Antibody Name
SCAPDL1xSCACD47
  Antibody Info 
Antigen Name
Programmed cell death ligand 1 (PD-L1); Leukocyte surface antigen CD47 (CD47)
  Antigen Info 
Payload Name
Active metabolite of irinotecan SN38
  Payload Info 
Therapeutic Target
DNA topoisomerase 1 (TOP1)
  Target Info 
Linker Name
30kmPEG-Lys (PEG2 - Mal)-3 (Val-Cit-PAB-DEA)
  Linker Info 
Conjugate Type
Random conjugation through reduced inter-chain cysteines.
Combination Type
30kmPEG-Lys (PEG2 - Mal)-3 (Val-Cit-PAB-DEA-SN38)
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 9 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Cell growth inhibition ratio 
≈ 20
%
CVCL_1577
Lung large cell carcinoma
Cell growth inhibition ratio 
≈ 22
%
CVCL_1566
Lung squamous cell carcinoma
Cell growth inhibition ratio 
≈ 25
%
CVCL_0186
Pancreatic ductal adenocarcinoma
Cell growth inhibition ratio 
≈ 35
%
CVCL_0186
Pancreatic ductal adenocarcinoma
Cell growth inhibition ratio 
≈ 40
%
CVCL_1577
Lung large cell carcinoma
Cell growth inhibition ratio 
≈ 42
%
CVCL_1566
Lung squamous cell carcinoma
Cell growth inhibition ratio 
≈ 65
%
CVCL_1566
Lung squamous cell carcinoma
Cell growth inhibition ratio 
≈ 70
%
CVCL_1577
Lung large cell carcinoma
Cell growth inhibition ratio 
≈ 75
%
CVCL_0186
Pancreatic ductal adenocarcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 9 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 20% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H661 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 10 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung large cell carcinoma NCI-H661 cells CVCL_1577
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 22% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H520 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 10 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung squamous cell carcinoma NCI-H520 cells CVCL_1566
Experiment 3 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 25% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line BXPC-3 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 10 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Pancreatic ductal adenocarcinoma BxPC-3 cells CVCL_0186
Experiment 4 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 35% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line BXPC-3 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 20 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Pancreatic ductal adenocarcinoma BxPC-3 cells CVCL_0186
Experiment 5 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 40% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H661 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 20 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung large cell carcinoma NCI-H661 cells CVCL_1577
Experiment 6 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 42% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H520 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 20 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung squamous cell carcinoma NCI-H520 cells CVCL_1566
Experiment 7 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 65% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H520 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 40 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung squamous cell carcinoma NCI-H520 cells CVCL_1566
Experiment 8 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 70% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line NCI-H661 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 40 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Lung large cell carcinoma NCI-H661 cells CVCL_1577
Experiment 9 Reporting the Activity Date of This ADC [1]
Efficacy Data Cell growth inhibition ratio ≈ 75% Positive CD47/PD-L1 expression (CD47/PD-L1+++/++)
Method Description
CD47/PD-L1-positive tumor cell line BXPC-3 cells were selected to be inoculated into 96-well plates with 3x105 cells/well and treated with the compound 89 with a dose of 40 ug/mL. Cell viability was measured by Cell Counting Kit-8 (CCK-8), and cell proliferation inhibition rate was calculated
In Vitro Model Pancreatic ductal adenocarcinoma BxPC-3 cells CVCL_0186
References
Ref 1 PEGylated antibody hydroxyl-bearing drug conjugates