Antibody-drug Conjugate Information
General Information of This Antibody-drug Conjugate (ADC)
| ADC ID |
DRG0RBWJG
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| ADC Name |
38703658 19G4-MMAE
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| Synonyms |
19G4-MMAE
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| Organization |
West China Hospital; West China Medical School; Sichuan University; State Key Laboratory of Biotherapy.
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| Drug Status |
Investigative
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| Antibody Name |
19G4
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Antibody Info | ||||
| Antigen Name |
Solute carrier family 3 member 2 (SLC3A2)
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Antigen Info | ||||
| Payload Name |
Monomethyl auristatin E
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Payload Info | ||||
| Therapeutic Target |
Microtubule (MT)
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Target Info | ||||
| Linker Name |
Undisclosed
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| Conjugate Type |
Site specific conjugation at LC-V205C.
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General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 2.028۪.4680 nM | Positive SLCA2 expression (SLCA2+++/++) | ||
| Method Description |
The MTT assay was performed to determin the in vitro efficacy of the 19G4-MMAE ADC. Cells were seeded in 96-well plates at a density of 5000 cells/well for SCC15, NPC/HK1, FADU, C666-1 at 37 °C and 5% CO2 overnight. Dilutions of PBS, 19G4, IgG-MMAE, and 19G4-MMAE solutions were added to cells for 72 h incubation at 37 °C. Cell viability were evaluated in triplicate by MTT assay. The IC50 was determined using Graphpad Prism version 9 (GraphPad Software, Inc.).
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| In Vitro Model | Squamous cell carcinoma of the oral tongue, Tongue squamous cell carcinoma | SCC15 cells | CVCL_1681 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 205.1 nM | Positive SLCA2 expression (SLCA2+++/++) | ||
| Method Description |
The MTT assay was performed to determin the in vitro efficacy of the 19G4-MMAE ADC. Cells were seeded in 96-well plates at a density of 5000 cells/well for SCC15, NPC/HK1, FADU, C666-1 at 37 °C and 5% CO2 overnight. Dilutions of PBS, 19G4, IgG-MMAE, and 19G4-MMAE solutions were added to cells for 72 h incubation at 37 °C. Cell viability were evaluated in triplicate by MTT assay. The IC50 was determined using Graphpad Prism version 9 (GraphPad Software, Inc.).
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| In Vitro Model | Nasopharyngeal carcinoma | NPC/HK1 cells | CVCL_7084 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 235.4 nM | Positive SLCA2 expression (SLCA2+++/++) | ||
| Method Description |
The MTT assay was performed to determin the in vitro efficacy of the 19G4-MMAE ADC. Cells were seeded in 96-well plates at a density of 5000 cells/well for SCC15, NPC/HK1, FADU, C666-1 at 37 °C and 5% CO2 overnight. Dilutions of PBS, 19G4, IgG-MMAE, and 19G4-MMAE solutions were added to cells for 72 h incubation at 37 °C. Cell viability were evaluated in triplicate by MTT assay. The IC50 was determined using Graphpad Prism version 9 (GraphPad Software, Inc.).
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| In Vitro Model | Hypopharyngeal squamous cell carcinoma | FaDu cells | CVCL_1218 | ||
| Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | 344.4 nM | Negative SLCA2 expression (SLCA2-) | ||
| Method Description |
The MTT assay was performed to determin the in vitro efficacy of the 19G4-MMAE ADC. Cells were seeded in 96-well plates at a density of 5000 cells/well for SCC15, NPC/HK1, FADU, C666-1 at 37 °C and 5% CO2 overnight. Dilutions of PBS, 19G4, IgG-MMAE, and 19G4-MMAE solutions were added to cells for 72 h incubation at 37 °C. Cell viability were evaluated in triplicate by MTT assay. The IC50 was determined using Graphpad Prism version 9 (GraphPad Software, Inc.).
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| In Vitro Model | Nasopharyngeal carcinoma | C666-1 cells | CVCL_7949 | ||
References