Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0PVJPH
ADC Name
IgG-NPLG-SN38
Synonyms
IgG-NPLG-SN38
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Organization
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education and Key Laboratory of Polymeric Materials & Application Technology of Hunan Province, Xiangtan University, Xiangtan, 411105, PR China; Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
Drug Status
Investigative
Structure
Antibody Name
undisclosed
Antigen Name
Undisclosed
Payload Name
Active metabolite of irinotecan SN38
  Payload Info 
Therapeutic Target
DNA topoisomerase 1 (TOP1)
  Target Info 
Linker Name
NPLG
  Linker Info 
Conjugate Type
Site-specific bioconjugation via Ultrahigh-DAR platform.
General Information of The ADMET Data Related to This ADC(2027 Update)
Excretion
Click To Hide/Show 1 Excretion Data Related to This Level
Standard Type Value Units Description Reference
Elimination Half-Life (t1/2) 7.3 h
NPLG-SN38/Cy7 was rapidly cleared from the plasma with a half-life (t1/2) of approximately 7.3 h
 [1]
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 1 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal inhibitory Concentration (lC50) 
> 100
uM
CVCL_B288
Mouse colon adenocarcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 1 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50) > 100 uM High PD-L1 expression (PD-L1 +++)
Method Description
The cytotoxicity of SN38, IgG-NPLG-SN38 and aPDL1-NPLG-SN38 toward MC38 cells was evaluated using a CCK-8 assay. Specifically, the MC38 cells were seeded into 96-well plates (5 × 103 cells per well) and incubated overnight. SN38, IgG-NPLG-SN38 or aPDL1-NPLG-SN38 was then added at a range of concentrations (0.01-100 uM SN38) to each well and cultured for 72 h. CCK-8 reagent was then added to each well and incubated for 1 h, after which the plate was measured on a microplate reader at a wavelength of 450 nm. Cell viability was calculated from the ratio of optical density (OD) values of sample to control wells.

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In Vitro Model Mouse colon adenocarcinoma MC38 cells CVCL_B288
References
Ref 1 Preparation of an Ultrahigh-DAR PDL1 monoclonal antibody-polymeric-SN38 conjugate for precise colon cancer therapy