Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)

ADC ID	DRG0FPEKT
ADC Name	MMAE-IgG1 3G12
Synonyms	MMAE IgG1 3G12; MMAE IgG1-3G12 Click to Show/Hide
Drug Status	Investigative
Indication	In total 1 Indication(s) Hepatoma [ICD11:2C12] Investigative
Drug-to-Antibody Ratio	2.28
	3D MOL	2D MOL
Antibody Name	Anti-ENPP1 IgG1 3G12		Antibody Info
Antigen Name	Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 (ENPP1)		Antigen Info
Payload Name	Monomethyl auristatin E		Payload Info
Therapeutic Target	Microtubule (MT)		Target Info
Linker Name	Undisclosed
Conjugate Type	NHS-lysine conjugation

General Information of The Activity Data Related to This ADC

Revealed Based on the Cell Line Data

Click To Hide/Show 3 Activity Data Related to This Level

Standard Type	Value	Units	Cell Line	Disease Model
Half Maximal Inhibitory Concentration (IC50)	1.00-10.00	nM	HEK293T cells (ENPP1 expression)	Hepatoma
Half Maximal Inhibitory Concentration (IC50)	> 100	nM	HEK293T cells	Normal
Half Maximal Inhibitory Concentration (IC50)	100	nM	Hep-G2 cells	Hepatoblastoma

Full List of Activity Data of This Antibody-drug Conjugate

Revealed Based on the Cell Line Data

Click To Hide/Show 3 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	1.00 nM-10.00 nM	Positive ENPP1 expression (ENPP1 +++/++)
Method Description	For the cell-killing activity of ADC,293T, 293T-ENPP1, and HepG2 cells were seeded at 2000 cells/well in 96-well white plate and incubated in cell growth medium overnight at 37°C in 5%CO₂.
In Vitro Model	Hepatoma	HEK293T cells (ENPP1 expression)		CVCL_0063
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	> 100 nM	Negative ENPP1 expression (ENPP1-)
Method Description	For the cell-killing activity of ADC,293T, 293T-ENPP1, and HepG2 cells were seeded at 2000 cells/well in 96-well white plate and incubated in cell growth medium overnight at 37°C in 5%CO₂.
In Vitro Model	Normal	HEK293T cells		CVCL_0063
Experiment 3 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	100 nM	Positive ENPP1 expression (ENPP1 +++/++)
Method Description	For the cell-killing activity of ADC,293T, 293T-ENPP1, and HepG2 cells were seeded at 2000 cells/well in 96-well white plate and incubated in cell growth medium overnight at 37°C in 5%CO₂.
In Vitro Model	Hepatoblastoma	Hep-G2 cells		CVCL_0027

References

Ref 1	Human antibodies targeting ENPP1 as candidate therapeutics for cancers. Front Immunol. 2023 Jan 25;14:1070492. doi: 10.3389/fimmu.2023.1070492. eCollection 2023.