Payload Information
General Information of This Payload
| Payload ID | PAY0TVGCR |
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| Name | ADC 1-4 payload |
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| Synonyms |
ADC1-4 payload
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| Target(s) | DNA topoisomerase 1 (TOP1) | |||||
Each Antibody-drug Conjugate Related to This Payload
Full Information of The Activity Data of The ADC(s) Related to This Payload
ADC1-4 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Maximum inhibition efficiency (MIE) |
20.12%
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Low PTK7 expression (PTK7 +) | ||
| Method Description |
Cells were then treated with a 10-points titration of compounds, including ADC1-1, ADC1-11,ADC1-4,ADC1-2 and ADC1-12. The plates were incubated for 7 days at 37°C, 5% CO2. Next, 50 uL of Cell-Titer- Glo reagent was added to each well. The mixture was mixed on an orbitalshaker for 10 mins to allow cell lysis.
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| In Vitro Model | Lung adenocarcinoma | A-549 cells | CVCL_0023 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Maximum inhibition efficiency (MIE) |
93.45%
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High PTK7 expression (PTK7 +++) | ||
| Method Description |
Cells were then treated with a 10-points titration of compounds, including ADC1-1, ADC1-11,ADC1-4,ADC1-2 and ADC1-12. The plates were incubated for 7 days at 37°C, 5% CO2. Next, 50 uL of Cell-Titer- Glo reagent was added to each well. The mixture was mixed on an orbitalshaker for 10 mins to allow cell lysis.
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| In Vitro Model | Ovarian serous adenocarcinoma | OVCAR-3 cells | CVCL_0465 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
0.32 nM
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High PTK7 expression (PTK7 +++) | ||
| Method Description |
Cells were then treated with a 10-points titration of compounds, including ADC1-1, ADC1-11,ADC1-4,ADC1-2 and ADC1-12. The plates were incubated for 7 days at 37°C, 5% CO2. Next, 50 uL of Cell-Titer- Glo reagent was added to each well. The mixture was mixed on an orbitalshaker for 10 mins to allow cell lysis.
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| In Vitro Model | Ovarian serous adenocarcinoma | OVCAR-3 cells | CVCL_0465 | ||
| Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) | > 200 nM | Low PTK7 expression (PTK7 +) | ||
| Method Description |
Cells were then treated with a 10-points titration of compounds, including ADC1-1, ADC1-11,ADC1-4,ADC1-2 and ADC1-12. The plates were incubated for 7 days at 37°C, 5% CO2. Next, 50 uL of Cell-Titer- Glo reagent was added to each well. The mixture was mixed on an orbitalshaker for 10 mins to allow cell lysis.
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| In Vitro Model | Lung adenocarcinoma | A-549 cells | CVCL_0023 | ||
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