Payload Information

General Information of This Payload

Payload ID	PAY0HFJPO
Name	Membrane-impermable colchinolmethyl ether (iCME) cytotoxin
Synonyms	Membrane-impermable colchinolmethyl ether (iCME) cytotoxin Click to Show/Hide
Target(s)	Microtubule (MT)	Target Info

Each Antibody-drug Conjugate Related to This Payload

Full Information of The Activity Data of The ADC(s) Related to This Payload

Trastuzumab-iCME [Investigative]

ADC Info

Revealed Based on the Cell Line Data

Click To Hide/Show 3 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	0.11 nM±0.07 nM	Positive HER2 expression (HER2 +++/++)
Method Description	To quantify cellular cytotoxicity, dose-response curves were generated with HER2-positive SKBR3 cells and HER2-negative MDA-MB-468 cells after treating with ADC and cholesterol-linked endosome-disruptive peptide (Chol-EDP) for 72 h.
In Vitro Model	Breast adenocarcinoma	SK-BR-3 cells		CVCL_0033
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	7.00 nM±5.00 nM	Positive HER2 expression (HER2 +++/++)
Method Description	To quantify cellular cytotoxicity, dose-response curves were generated with HER2-positive SKBR3 cells and HER2-negative MDA-MB-468 cells after treating with ADC for 72 h.
In Vitro Model	Breast adenocarcinoma	SK-BR-3 cells		CVCL_0033
Experiment 3 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	14.00 nM±8.00 nM	Negative HER2 expression (HER2 -)
Method Description	To quantify cellular cytotoxicity, dose-response curves were generated with HER2-positive SKBR3 cells and HER2-negative MDA-MB-468 cells after treatment for 72 h.
In Vitro Model	Breast adenocarcinoma	MDA-MB-468 cells		CVCL_0419

References

Ref 1	Antibody-Drug Conjugate that Exhibits Synergistic Cytotoxicity with an Endosome-Disruptive Peptide. ACS Omega. 2019 Jul 31;4(7):12955-12968. doi: 10.1021/acsomega.9b01585. eCollection 2019 Jul 31.