Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0RNZTQ |
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| Antibody Name | Ab4 |
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| Organization | ELI LILLY AND COMPANY |
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| Synonyms |
Ab4
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| Antibody Type | Monoclonal antibody (mAb) |
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| Antibody Subtype | Humanized lgG |
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| Antigen Name | Nectin-4 (NECTIN4) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
| Heavy Chain Sequence |
QVQLQESGPGLVKPSETLSLTCTVSGGSISGYYWSWIRQPPGKGLEWIGYIYYSGSTNYN
PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARLGIFFDAFDIWGQGTLVTVSSAS TKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGL YSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPEAAGGPS VFLFPPKPKDTLMISRTPEVTCVVVSVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNST YRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMT KNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQ GNVFSCSVMHEALHNHYTQKSLSLSPGK Click to Show/Hide
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| Heavy Chain Varible Domain |
QVQLQESGPGLVKPSETLSLTCTVSGGSISGYYWSWIRQPPGKGLEWIGYIYYSGSTNYN
PSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARLGIFFDAFDIWGQGTLVTVSS Click to Show/Hide
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| Heavy Chain CDR 1 |
TVSGGSISGYYWS
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| Heavy Chain CDR 2 |
YIYYSGSTN
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| Heavy Chain CDR 3 |
ARLGIFFDAFDI
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| Light Chain Sequence |
DIQLTQSPSFLSASVGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
RFSGSGSGTEFTLTISSLQPEDFATYYCQQFNSYPWTFGQGTKVEIKRTVAAPSVFIFPP SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT LSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC Click to Show/Hide
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| Light Chain Varible Domain |
DIQLTQSPSFLSASVGDRVTITCRASQGISSYLAWYQQKPGKAPKLLIYAASTLQSGVPS
RFSGSGSGTEFTLTISSLQPEDFATYYCQQFNSYPWTFGQGTKVEIK Click to Show/Hide
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| Light Chain CDR 1 |
RASQGISSYLA
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| Light Chain CDR 2 |
YAASTLQS
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| Light Chain CDR 3 |
QQFNSYPWT
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The Activity Data of This Antibody
| Antibody Activity Information 1 | [1] | |||||
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Half Maximal Effective Concentration (EC50)
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0.14
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nM
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NCI-H1781 cells | CVCL_1494 | ||
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| Antigen Expression | Positive Tissue factor expression (TF+++/++; >350,000 tissue factor molecules/cell) | |||||
| Antibody Function | Ab4 bound to NCI-H1781 cells | |||||
| Antibody Antigen Binding Assay | NCI-H1781 tumor cells representing lower endogenous expression. Antibody binding was quantified via flow cytometry and both the EC50 of the binding curves. | |||||
| Antibody Activity Information 2 | [1] | |||||
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Half Maximal Effective Concentration (EC50)
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0.19
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nM
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SUM190PT tumor cells | CVCL_3423 | ||
| Antigen Expression | Positive Tissue factor expression (TF+++/++; >350,000 tissue factor molecules/cell) | |||||
| Antibody Function | Ab4 bound to SUM190PT tumor cells | |||||
| Antibody Antigen Binding Assay | SUM190PT tumor cells representing high endogenous expression. Antibody binding was quantified via flow cytometry and both the EC50 of the binding curves. | |||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
JP2025011033A Ab4 [Investigative]
Discovered Using Cell Line-derived Xenograft Model
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Mean tumor colume±SEM |
343.65䕈.11 mm3
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Moderate nectin-4 expression (nectin-4++) | ||
| Method Description |
Female NSG mice between 5-6 weeks of age were injected subcutaneously withMDA-MB-468 cells with moderate nectin-4 expression, when tumors reached appproximately 150-250 mm^3, animals were matched by tumor volume into treatment or controls groups and dosing initiated (Day 0, n=8 per group). The test articles were given at a single dose (2 mg/kg) formulated with 5% dextrose. Tumors were measured biweekly until Day 71.
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| In Vivo Model | MDAMB468 xenograft model | ||||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Mean tumor colume±SEM |
928.09𫏏.94 mm3
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High nectin-4 expression (nectin-4 +++) | ||
| Method Description |
Immune compromised female mice between 5-8 weeks of age with weight 18-20 grams were implanted unilaterally on the right flank with UM-UC-3 Nectin4 clone F7 cells with high nectin-4 expression, when tumors reached appproximately 150-250 mm^3, animals were matched by tumor volume into treatment or controls groups and dosing initiated (Day 0, n=8 per group). The test articles were given at a single dose (2 mg/kg) formulated with 5% dextrose. Tumors were measured biweekly until Day 40.
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| In Vivo Model | UMUC3 Nectin-4 clone F7 xenograft model | ||||
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
0.1154 nM
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Positive nectin-4 expression (nectin-4+++/++) | ||
| Method Description |
ADCs were tested in T24-human nectin-4 clone 108 cells, and were added at final working concentration from 200 nM, 1:4 serially diluted in culture medium for 5 days.
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| In Vitro Model | Bladder carcinoma | T24-human nectin-4 clone cells | CVCL_0554 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
0.3107 nM
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Positive nectin-4 expression (nectin-4+++/++) | ||
| Method Description |
ADCs were tested in NCI H1781 cells, and were added at final working concentration from 200 nM, 1:4 serially diluted in culture medium for 5 days.
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| In Vitro Model | Minimally invasive lung adenocarcinoma | NCI-H1781 cells | CVCL_1494 | ||