All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.

All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.

All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.

All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.

All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.

All cell lines were cultured at 37oC in a humidified atmosphere in the presence of 5% CO2 and 95% air in Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% FBS containing high glucose (4500 mg/liter) and antibiotics (penicillin 100 U/ml, streptomycin 100 ug/ml). Cells were tested for authenticity by STR at the Hematology Service of the University of Salamanca. NP29 and NP31 cells were obtained from patient samples by Dr. Gabriel Capellá. Cell proliferation experiments were conducted using a Z1 Coulter Particle Counter as described previously [28, 29]. Apoptosis and cell cycle analyses were performed as described [30]. The KRAS mutation status of genomic DNA isolated using TRIzol (Thermo Fisher Scientific) from Capan-1, IMIM-PC1, IMIM-PC2, SKPC1, NP29, and NP31 cell lines was analyzed with the KRAS Mutation Test v2 (LSR) on the Cobas® platform (Roche Diagnostics) by PCR using the Cobas® z 480 analyzer (Roche). The resulting data are presented in Supplementary Table 1.