Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0LQGRT |
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| Antibody Name | Tra-CAST |
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| Organization | Fudan University.; Westlake Laboratory of Life Sciences and Biomedicine.; Westlake University.; Westlake Institute for Advanced Study.; Zhejiang University.; University of California at San Francisco. |
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| Synonyms |
Tra-CAST
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| Antibody Type | Monoclonal antibody (mAb) |
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| Antibody Subtype | Humanized lgG1 |
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| Antigen Name | Receptor tyrosine-protein kinase erbB-2 (ERBB2) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
Tra-CAST-MMAE [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
0.2 nM
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High HER2 expression (HER2 +++) | ||
| Method Description |
Cells were seeded in a 96-well white opaque plate at a density of 5 × 103/well (CHO, ATCC, CCL-61) or 1 × 104/well (MCF7 (ATCC, HTB-22), SK-BR-3 (ATCC, HTB-30), SK-OV-3 (ATCC, HTB-77) or JIMT-1 (Beyotime, C6453)). Cells were allowed to attach for 24 h at 37 °C and 5% CO2 in humidified atmosphere. Cells were then treated with serial dilutions of Tra-CAST, Tra-CAST-MMAE and SBA-MMAE or Tra-CASTi, Tra-CASTi-MMAE and DBCO-MMAE for 96 h (BT474, MCF7, SK-BR-3). Cell viability was determined using CellTiter Glo reagents (G7571) and was normalized to the control cells. Data was analyzed by Graphpad software, and the half-maximal effective concentration (EC50) value was calculated by fitting with the log (inhibitor) vs. response module.
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| In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
1.2 nM
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High HER2 expression (HER2 +++) | ||
| Method Description |
Cells were seeded in a 96-well white opaque plate at a density of 5 × 103/well (CHO, ATCC, CCL-61) or 1 × 104/well (MCF7 (ATCC, HTB-22), SK-BR-3 (ATCC, HTB-30), SK-OV-3 (ATCC, HTB-77) or JIMT-1 (Beyotime, C6453)). Cells were allowed to attach for 24 h at 37 °C and 5% CO2 in humidified atmosphere. Cells were then treated with serial dilutions of Tra-CAST, Tra-CAST-MMAE and SBA-MMAE or Tra-CASTi, Tra-CASTi-MMAE and DBCO-MMAE for 96 h (BT474, MCF7, SK-BR-3). Cell viability was determined using CellTiter Glo reagents (G7571) and was normalized to the control cells. Data was analyzed by Graphpad software, and the half-maximal effective concentration (EC50) value was calculated by fitting with the log (inhibitor) vs. response module.
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| In Vitro Model | Ovarian serous adenocarcinoma | SKOV-3 cells | CVCL_0532 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
1.7 nM
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Positive HER2 expression ( HER2+++/++) | ||
| Method Description |
Cells were seeded in a 96-well white opaque plate at a density of 5 × 103/well (CHO, ATCC, CCL-61) or 1 × 104/well (MCF7 (ATCC, HTB-22), SK-BR-3 (ATCC, HTB-30), SK-OV-3 (ATCC, HTB-77) or JIMT-1 (Beyotime, C6453)). Cells were allowed to attach for 24 h at 37 °C and 5% CO2 in humidified atmosphere. Cells were then treated with serial dilutions of Tra-CAST, Tra-CAST-MMAE and SBA-MMAE or Tra-CASTi, Tra-CASTi-MMAE and DBCO-MMAE for 96 h (BT474, MCF7, SK-BR-3). Cell viability was determined using CellTiter Glo reagents (G7571) and was normalized to the control cells. Data was analyzed by Graphpad software, and the half-maximal effective concentration (EC50) value was calculated by fitting with the log (inhibitor) vs. response module.
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| In Vitro Model | Breast ductal carcinoma | JIMT-1 cells | CVCL_2077 | ||
References