The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.

The Cell Counting Kit-8 (CCK-8) assay was performed to measure the in vitro efficiency of ADCs. TROP2-expression tumor cell lines (BXPC-3, HCI-441, Fadu, HCC1806, NCI-N87) and TROP2 negative tumor cell lines (Calu-6) were cultured in a 10 % FBS-containing RPMI 1640 medium and were planted into 96-well plates with 6000 cells per well. The plates were incubated overnight at 37 °C in a 5 % CO2 cell incubator. All ADCs were diluted 5-fold with RPMI 1640 medium from an initial concentration of 100 nmol/L, a total of nine concentration gradients. The ADC samples were added to three wells with every single concentration and the 96-well plates were cultured at 37 °C in a 5 % CO2 cell incubator for six days. Then CCK8 solution was added and incubated at 37 °C for 4 h. Optical density (OD) value was measured at 450 nm using a BioTek Epoch. IC50 values and the cell viability curve were calculated by GraphPad software.