Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0ADWPG |
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| Antibody Name | B7-H3 mAb 401 |
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| Organization | shanghai Institute for Advanced Immunochemical Studies ShanghaiTech University. |
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| Synonyms |
B7-H3 mAb 401
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| Antibody Type | Monoclonal antibody (mAb) |
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| Antibody Subtype | Humanized lgG |
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| Antigen Name | CD276 antigen (CD276) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
37235999 ADC 401-4 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
13.18 nM
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High B7-H3 expression (B7-H3 +++) | ||
| Method Description |
Cancer cell lines U87, U251, U87/B7-H3 KO cells (3000 cells per well) at logarithmic growth stage were seeded in 96-well plates and allowed to adhere overnight at 37 °C in a humidified incubator of 5% CO2. After that, ADCs, 401 and MMAE were diluted at a certain concentration gradient and added to cell plates in triplicate, the cells were treated at 37 °C with 5% CO2 for 72 h. In addition, the complete medium without cells served as the blank and the cells cultured in medium alone served as vehicle control.
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| In Vitro Model | Glioblastoma | U87 cells | CVCL_0022 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
35.48 nM
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High B7-H3 expression (B7-H3 +++) | ||
| Method Description |
Cancer cell lines U87, U251, U87/B7-H3 KO cells (3000 cells per well) at logarithmic growth stage were seeded in 96-well plates and allowed to adhere overnight at 37 °C in a humidified incubator of 5% CO2. After that, ADCs, 401 and MMAE were diluted at a certain concentration gradient and added to cell plates in triplicate, the cells were treated at 37 °C with 5% CO2 for 72 h. In addition, the complete medium without cells served as the blank and the cells cultured in medium alone served as vehicle control.
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| In Vitro Model | Astrocytoma | U251 cells | CVCL_0021 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
239.88 nM
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High B7-H3 expression (B7-H3 +++) | ||
| Method Description |
Cancer cell lines U87, U251, U87/B7-H3 KO cells (3000 cells per well) at logarithmic growth stage were seeded in 96-well plates and allowed to adhere overnight at 37 °C in a humidified incubator of 5% CO2. After that, ADCs, 401 and MMAE were diluted at a certain concentration gradient and added to cell plates in triplicate, the cells were treated at 37 °C with 5% CO2 for 72 h. In addition, the complete medium without cells served as the blank and the cells cultured in medium alone served as vehicle control.
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| In Vitro Model | Glioblastoma | U87 cells (B7-H3 KO) | CVCL_0022 | ||
References