Antibody Information

General Information of This Antibody

Each Antibody-drug Conjugate Related to This Antibody

Full Information of The Activity Data of The ADC(s) Related to This Antibody

HN3-PE24 [Investigative]

ADC Info

Discovered Using Cell Line-derived Xenograft Model

Click To Hide/Show 1 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Tumor Growth Inhibition value (TGI)	≈ 63.30% (Day 48)	Positive GPC3 expression (GPC3+++/++)
Method Description	HN3-PE24 induces efficient tumor cell killing in cell line-derived models of immunotherapy of hepatocellular carcinoma cells.
In Vivo Model	Hepatocellular carcinoma CDX model
In Vitro Model	Hepatocellular carcinoma	Hepatocellular carcinoma cells		Homo sapiens

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Inhibitory Concentration 5% (IC5)	110.00 ng/mL	Positive GPC3 expression (GPC3+++/++)
Method Description	Ten thousand cells were seeded in a 96-well cell culture plate (200 l per well), supplemented with purified immunotoxins at the indicated final concentrations, and incubated at 37°C for 3 days. Cell viability was determined by quantifying the enzymatic activity of the intracellular luciferase that was released by two rounds of freezing-thawing. Click to Show/Hide
In Vitro Model	Hepatocellular carcinoma	G1LG cells		Homo sapiens
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	110.00 ng/mL	Positive GPC3 expression (GPC3+++/++)
Method Description	Ten thousand cells were seeded in a 96-well cell culture plate (200 l per well), supplemented with purified immunotoxins at the indicated final concentrations, and incubated at 37°C for 3 days. Cell viability was determined by quantifying the enzymatic activity of the intracellular luciferase that was released by two rounds of freezing-thawing. Click to Show/Hide
In Vitro Model	Pediatric hepatocellular carcinoma	Hep3BLG cells		Homo sapiens

References

Ref 1	Highly Potent Immunotoxins Targeting the Membrane-distal N-lobe of GPC3 for Immunotherapy of Hepatocellular Carcinoma. J Cancer. 2022 Feb 14;13(4):1370-1384.