Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)

ADC ID	DRG0SMKOP
ADC Name	Anti-CD70 mAb 1F4-IIIc-08
Synonyms	Anti-CD70-mAb-1F4-IIIc-08 Click to Show/Hide
Organization	Bristol Myers Squibb Co.
Drug Status	Investigative
Indication	In total 1 Indication(s) Renal cell carcinoma [ICD11:2C90] Investigative
Drug-to-Antibody Ratio	2.3
	3D MOL	2D MOL
Antibody Name	Anti-CD70 mAb 1F4		Antibody Info
Antigen Name	CD70 antigen (CD70)		Antigen Info
Payload Name	Undisclosed
Linker Name	Anti-CD70 mAb 1F4-IIIc-08 linker
Conjugate Type	Random conjugation through reduced inter-chain cysteines.

General Information of The Activity Data Related to This ADC

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Standard Type	Value	Units	Cell Line	Disease Model
Half Maximal Inhibitory Concentration (IC50)	0	nM	CVCL_1603	Gastric tubular adenocarcinoma
Half Maximal Inhibitory Concentration (IC50)	0.02	nM	CVCL_1051	Renal cell carcinoma

Full List of Activity Data of This Antibody-drug Conjugate

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	1.20 nM	Negative CD70 expression (CD70-)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Gastric tubular adenocarcinoma	NCI-N87 cells		CVCL_1603
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	0.02 nM	Positive CD70 expression (CD70+++/++)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Renal cell carcinoma	786-O cells		CVCL_1051

References

Ref 1	Benzodiazepine dimers, conjugates thereof, and methods of making and using.