Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)

ADC ID	DRG0PLRAG
ADC Name	Anti-MSLN mAb 6A4-IIIa-05
Synonyms	Anti-MSLN-mAb-6A4-IIIa-05 Click to Show/Hide
Organization	Bristol Myers Squibb Co.
Drug Status	Investigative
Indication	In total 1 Indication(s) Gastric cancer [ICD11:2B72] Investigative
Drug-to-Antibody Ratio	3
	3D MOL	2D MOL
Antibody Name	Anti-MSLN mAb 6A4		Antibody Info
Antigen Name	Mesothelin (MSLN)		Antigen Info
Payload Name	Undisclosed
Linker Name	Anti-MSLN mAb 6A4-IIIa-05 linker
Conjugate Type	Random conjugation through nucleophilic lysines.

General Information of The Activity Data Related to This ADC

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Standard Type	Value	Units	Cell Line	Disease Model
Half Maximal Effective Concentration (EC50)	0.01	nM	CVCL_1544	Pleural epithelioid mesothelioma
Half Maximal Effective Concentration (EC50)	0.04	nM	CVCL_1603	Gastric tubular adenocarcinoma

Full List of Activity Data of This Antibody-drug Conjugate

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Effective Concentration (EC50)	0.01 nM	Positive MSLN expression (MSLN+++/++)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Pleural epithelioid mesothelioma	NCI-H226 cells		CVCL_1544
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Effective Concentration (EC50)	0.04 nM	Positive MSLN expression (MSLN+++/++)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Gastric tubular adenocarcinoma	NCI-N87 cells		CVCL_1603

References

Ref 1	Benzodiazepine dimers, conjugates thereof, and methods of making and using.