Antibody-drug Conjugate Information
General Information of This Antibody-drug Conjugate (ADC)
| ADC ID |
DRG0NVLCZ
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| ADC Name |
121G12-DAPA-sSPDB-DM4
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| Synonyms |
121G12 DAPA sSPDB DM4
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| Organization |
Novartis AG
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| Drug Status |
Investigative
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| Indication |
In total 3 Indication(s)
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| Drug-to-Antibody Ratio |
3.6
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| Antibody Name |
Anti-CCR7 mAb 121G12
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Antibody Info | ||||
| Antigen Name |
C-C chemokine receptor type 7 (CCR7)
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Antigen Info | ||||
| Payload Name |
Undisclosed
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| Linker Name |
121G12.DAPA.sSPDB.DM4 linker
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General Information of The Activity Data Related to This ADC
Discovered Using Cell Line-derived Xenograft Model
Revealed Based on the Cell Line Data
Full List of Activity Data of This Antibody-drug Conjugate
Discovered Using Cell Line-derived Xenograft Model
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Tumor Growth Inhibition value (TGI) | ≌ 13.77% (Day 9) | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
All treatments were conducted at day 0 by a single dose, i.v, 2 mg/kg x1 injection into the tail vein, Data, depicted as mean tumour volume, consists of 6-8 animals per experimental group.
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| In Vivo Model | KE97 CDX model | ||||
| In Vitro Model | Normal | KE-97 cells | CVCL_3386 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Tumor Growth Inhibition value (TGI) | ≍ 33.00% (Day 9) | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
All treatments were conducted at day 0 by a single dose, i.v, 5 mg/kg x1 injection into the tail vein, Data, depicted as mean tumour volume, consists of 6-8 animals per experimental group.
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| In Vivo Model | KE97 CDX model | ||||
| In Vitro Model | Normal | KE-97 cells | CVCL_3386 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Tumor Growth Inhibition value (TGI) | ≋ 85.38% (Day 9) | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
All treatments were conducted at day 0 by a single dose, i.v, 0.5 mg/kg x1 injection into the tail vein, Data, depicted as mean tumour volume, consists of 6-8 animals per experimental group.
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| In Vivo Model | KE97 CDX model | ||||
| In Vitro Model | Normal | KE-97 cells | CVCL_3386 | ||
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | 2.98 nM | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
Target expressing or non-expressing cells were seeded in 96-well cell culture plates for 24 hours before treatment. Cells were treated with 3-fold serially diluted antibody-drug conjugates or free compounds (i.e, no antibody conjugated to the drug) in duplicate at 10 concentrations. Cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation MTS Assay.
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| In Vitro Model | Anaplastic large cell lymphoma | DEL cells | CVCL_1170 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | 3.18 nM | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
Target expressing or non-expressing cells were seeded in 96-well cell culture plates for 24 hours before treatment. Cells were treated with 3-fold serially diluted antibody-drug conjugates or free compounds (i.e, no antibody conjugated to the drug) in duplicate at 10 concentrations. Cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation MTS Assay.
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| In Vitro Model | Normal | KE-97 cells | CVCL_3386 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | 3.91 nM | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
Target expressing or non-expressing cells were seeded in 96-well cell culture plates for 24 hours before treatment. Cells were treated with 3-fold serially diluted antibody-drug conjugates or free compounds (i.e, no antibody conjugated to the drug) in duplicate at 10 concentrations. Cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation MTS Assay.
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| In Vitro Model | Hodgkin lymphoma | L-540 cells | CVCL_1362 | ||
| Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | 8.42 nM | Positive CCR7 expression (CCR7+++/++) | ||
| Method Description |
Target expressing or non-expressing cells were seeded in 96-well cell culture plates for 24 hours before treatment. Cells were treated with 3-fold serially diluted antibody-drug conjugates or free compounds (i.e, no antibody conjugated to the drug) in duplicate at 10 concentrations. Cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation MTS Assay.
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| In Vitro Model | Anaplastic large cell lymphoma | DEL cells | CVCL_1170 | ||