Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0MPUPN
ADC Name
Anti-MSLN mAb 6A4-IIIa-06
Synonyms
Anti-MSLN-mAb-6A4-IIIa-06
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Organization
Bristol Myers Squibb Co.
Drug Status
Investigative
Indication
In total 1 Indication(s)
Gastric cancer [ICD11:2B72]
Investigative
Drug-to-Antibody Ratio
3
Antibody Name
Anti-MSLN mAb 6A4
  Antibody Info 
Antigen Name
Mesothelin (MSLN)
  Antigen Info 
Payload Name
Undisclosed
Linker Name
Anti-MSLN mAb 6A4-IIIa-06 linker
Conjugate Type
Random conjugation through nucleophilic lysines.
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 3 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal Effective Concentration (EC50) 
0.01
nM
CVCL_1544
Pleural epithelioid mesothelioma
Half Maximal Effective Concentration (EC50) 
0.03
nM
CVCL_1603
Gastric tubular adenocarcinoma
Half Maximal Effective Concentration (EC50) 
45
nM
CVCL_1051
Renal cell carcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 3 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 0.01 nM Positive MSLN expression (MSLN+++/++)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Pleural epithelioid mesothelioma NCI-H226 cells CVCL_1544
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 0.03 nM Positive MSLN expression (MSLN+++/++)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Gastric tubular adenocarcinoma NCI-N87 cells CVCL_1603
Experiment 3 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 45.00 nM Negative MSLN expression (MSLN-)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Renal cell carcinoma 786-O cells CVCL_1051
References
Ref 1 Benzodiazepine dimers, conjugates thereof, and methods of making and using.

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