Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)

ADC ID	DRG0KVVMV
ADC Name	Anti-GPC3 mAb 4A6-IIIb-01
Synonyms	Anti-GPC3-mAb-4A6-IIIb-01 Click to Show/Hide
Organization	Bristol Myers Squibb Co.
Drug Status	Investigative
Indication	In total 1 Indication(s) Hepatocellular carcinoma [ICD11:2C12] Investigative
Drug-to-Antibody Ratio	2.7
	3D MOL	2D MOL
Antibody Name	Anti-GPC3 mAb 4A6		Antibody Info
Antigen Name	Glypican-3 (GPC3)		Antigen Info
Payload Name	Undisclosed
Linker Name	Anti-GPC3 mAb 4A6-IIIb-01 linker
Conjugate Type	Random conjugation through reduced inter-chain cysteines.

General Information of The Activity Data Related to This ADC

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Standard Type	Value	Units	Cell Line	Disease Model
Half Maximal Inhibitory Concentration (IC50)	228	pM	CVCL_0326	Childhood hepatocellular carcinoma
Half Maximal Inhibitory Concentration (IC50)	0	nM	CVCL_1603	Gastric tubular adenocarcinoma

Full List of Activity Data of This Antibody-drug Conjugate

Revealed Based on the Cell Line Data

Click To Hide/Show 2 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	228.00 pM	Positive GPC3 expression (GPC3+++/++)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Childhood hepatocellular carcinoma	Hep 3B2.1-7 cells		CVCL_0326
Experiment 2 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	14.00 nM	Negative GPC3 expression (GPC3-)
Method Description	The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO₂ before assaying. Click to Show/Hide
In Vitro Model	Gastric tubular adenocarcinoma	NCI-N87 cells		CVCL_1603

References

Ref 1	Benzodiazepine dimers, conjugates thereof, and methods of making and using.