In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.

In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.

In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.

In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.

In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.

In vitro activity evaluation mainly included antitumor cell proliferation, apoptosis, cell cycle, anticell migration, and in vitro antiangiogenesis activity evaluation. In this study, HT-29, A549, and HepG2 cells were selected as experimental cell lines to evaluate the antitumor activity in vitro. The antiproliferative activity of ADC was evaluated based on the CCK-8 method, with the concentration range set to 0-10 ug/mL, and the illumination time set to 15 min. Annexin V-FITC/PI double staining and technique. The concentration was 10 ug/mL at the initial screening, the concentration range was 0-20 ug/mL in a concentration-dependent setting, and the light time was 15 min. The cell cycle arrest activity was assessed using PI staining. The concentration was 5 ug/mL at the initial screening, the concentration range was 0-10 ug/mL in a concentration-dependent study, and the illumination time was 15 min. In the evaluation of anticell migration activity, the concentration of ADC was set to 10 ug/mL, and the illumination time was set to 15 min. The evaluation of antiangiogenesis activity in vitro included assessing the toxicity of ADC to HUVEC cells and evaluating anti-HUVEC cell migration activity. Cytotoxicity was evaluated using the CCK-8 method and the Ca-AM/PI method, respectively. In the evaluation of anti-HUVEC cell migration activity, the ADC concentration was set at 10 ug/mL.