Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0EPZKH
ADC Name
ICT_betaGlu-EXA-2
Synonyms
ICT_betaGlu-EXA-2
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Organization
Emergence Therapeutics AG
Drug Status
Investigative
Drug-to-Antibody Ratio
7.89
Antibody Name
huASCT2 17c10 mAb
  Antibody Info 
Antigen Name
Amino acid transporter (ASCT2)
  Antigen Info 
Payload Name
Undisclosed
Therapeutic Target
Stimulator of interferon genes protein (STING1)
  Target Info 
Linker Name
Undisclosed
Combination Type
EXA-2
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 2 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal inhibitory Concentration (lC50) 
40- 1.53 × 10 -4
ug/mL
CVCL_2760
Colon adenocarcinoma
Half Maximal inhibitory Concentration (lC50) 
80- 3.05 × 10 -4
ug/mL
CVCL_0291
Colon carcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 2 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50) 40- 1.53 × 10 -4 ug/mL Positive ASCT2 expression (ASCT2+++/++)
Method Description
Target cells were seeded in triplicates into flat-bottom 384-wells plate (400 cells for HCT116 cells). Cells were incubated for 3 to 4 hours at 37°C before addition of an equivalent volume of 2X concentrated ADC or free drug-linker dose range (see table 1 for details). Cells were then incubated into a live cell imaging instrument (Incucyte®) for 6 to 8 days and their confluency was monitored all along the culture.

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In Vitro Model Colon adenocarcinoma WiDr cells CVCL_2760
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal inhibitory Concentration (lC50) 80- 3.05 × 10 -4 ug/mL Positive ASCT2 expression (ASCT2+++/++)
Method Description
Target cells were seeded in triplicates into flat-bottom 384-wells plate (400 cells for HCT116 cells). Cells were incubated for 3 to 4 hours at 37°C before addition of an equivalent volume of 2X concentrated ADC or free drug-linker dose range (see table 1 for details). Cells were then incubated into a live cell imaging instrument (Incucyte®) for 6 to 8 days and their confluency was monitored all along the culture.

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In Vitro Model Colon carcinoma HCT116 cells CVCL_0291
References
Ref 1 Anti-ASCT2 antibodies and ADCs derived therefrom