Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0CRUZX
ADC Name
Anti-CD70 mAb 1F4-IIIb-03
Synonyms
Anti-CD70-mAb-1F4-IIIb-03
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Organization
Bristol Myers Squibb Co.
Drug Status
Investigative
Indication
In total 1 Indication(s)
Renal cell carcinoma
Investigative
.
Drug-to-Antibody Ratio
1
Antibody Name
Anti-CD70 mAb 1F4
  Antibody Info 
Antigen Name
CD70 antigen (CD70)
  Antigen Info 
Payload Name
Undisclosed
Linker Name
Anti-CD70 mAb 1F4-IIIb-03 linker
Conjugate Type
Random conjugation through reduced inter-chain cysteines.
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 2 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal Inhibitory Concentration (IC50) 
0
nM
CVCL_1603
Gastric tubular adenocarcinoma
Half Maximal Inhibitory Concentration (IC50) 
0.06
nM
CVCL_1051
Renal cell carcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 2 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Inhibitory Concentration (IC50) 6.70 nM Negative CD70 expression (CD70-)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Gastric tubular adenocarcinoma NCI-N87 cells CVCL_1603
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Inhibitory Concentration (IC50) 0.06 nM Positive CD70 expression (CD70+++/++)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Renal cell carcinoma 786-O cells CVCL_1051
References
Ref 1 Benzodiazepine dimers, conjugates thereof, and methods of making and using.