Antibody-drug Conjugate Information

General Information of This Antibody-drug Conjugate (ADC)
ADC ID
DRG0ASJYK
ADC Name
Anti-FucGM1 mAb-IIIa-06
Synonyms
Anti-FucGM1-mAb-IIIa-06
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Organization
Bristol Myers Squibb Co.
Drug Status
Investigative
Indication
In total 1 Indication(s)
Small cell lung cancer
Investigative
.
Drug-to-Antibody Ratio
2
Antibody Name
Anti-FucGM1 mAb
  Antibody Info 
Antigen Name
Fucosylated monosialotetrahexosylganglioside (FucGM1)
  Antigen Info 
Payload Name
Undisclosed
Linker Name
Anti-FucGM1 mAb linker
Conjugate Type
Random conjugation through nucleophilic lysines.
General Information of The Activity Data Related to This ADC
Revealed Based on the Cell Line Data
Click To Hide/Show 3 Activity Data Related to This Level
Standard Type Value Units Cell Line Disease Model
Half Maximal Effective Concentration (EC50) 
4
nM
CVCL_1544
Pleural epithelioid mesothelioma
Half Maximal Effective Concentration (EC50) 
6.9
nM
CVCL_1603
Gastric tubular adenocarcinoma
Half Maximal Effective Concentration (EC50) 
44
nM
CVCL_1051
Renal cell carcinoma
Full List of Activity Data of This Antibody-drug Conjugate
Revealed Based on the Cell Line Data
Click To Hide/Show 3 Activity Data Related to This Level
Experiment 1 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 4.00 nM Negative FucGM1 expression (FucGM1-)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Pleural epithelioid mesothelioma NCI-H226 cells CVCL_1544
Experiment 2 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 6.90 nM Negative FucGM1 expression (FucGM1-)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Gastric tubular adenocarcinoma NCI-N87 cells CVCL_1603
Experiment 3 Reporting the Activity Date of This ADC [1]
Efficacy Data Half Maximal Effective Concentration (EC50) 44.00 nM Negative FucGM1 expression (FucGM1-)
Method Description
The MTS cell proliferation assay was performed as follows: CellTiter 96 Aqueous Non-Radioactive Cell proliferation Kit is used to determine the number of viable cells in cell proliferation assay. Tumor cells are plated at certain seeding densities in sterile 384-well black clear bottom Matrix plates at 40 uL per well and incubated overnight at 37°C in 5% CO2 before assaying.

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In Vitro Model Renal cell carcinoma 786-O cells CVCL_1051
References
Ref 1 Benzodiazepine dimers, conjugates thereof, and methods of making and using.