Payload Information

General Information of This Payload

Payload ID	PAY0NNLXO
Name	RSL3-NH2
Synonyms	RSL3-NH2 Click to Show/Hide
Target(s)	Phospholipid hydroperoxide glutathione peroxidase (GPX4)			Target Info
Structure
Structure	3D MOL		2D MOL
Formula	C21H20ClN3O3
Isosmiles	COC(=O)C1Cc2c([nH]c3ccccc23)C(c2ccc(N)cc2)N1C(=O)CCl
InChI	InChI=1S/C21H20ClN3O3/c1-28-21(27)17-10-15-14-4-2-3-5-16(14)24-19(15)20(25(17)18(26)11-22)12-6-8-13(23)9-7-12/h2-9,17,20,24H,10-11,23H2,1H3
InChIKey	MILRUPZPRGKLNY-UHFFFAOYSA-N
Pharmaceutical Properties	Molecule Weight	397.862	Polar area	88.42
	Complexity	28	xlogp Value	3.0046
	Heavy Count	28	Rot Bonds	3
	Hbond acc	4	Hbond Donor	2

The activity data of This Payload

Standard Type	Value	Units	Cell line	Disease Model	Cell line ID	Reference
Half Maximal Inhibitory Concentration (IC50)	10	nM	BT-474 cells	Invasive breast carcinoma	CVCL_0179	[1]
Half Maximal Inhibitory Concentration (IC50)	10	nM	HEL cells	Erythroleukemia	CVCL_0001	[1]
Half Maximal Inhibitory Concentration (IC50)	1000	nM	Karpas-422 cells	Diffuse large B-cell lymphoma	CVCL_1325	[1]
Half Maximal Inhibitory Concentration (IC50)	>1000	nM	NCI-N87 cells	Gastric tubular adenocarcinoma	CVCL_1603	[1]
Half Maximal Inhibitory Concentration (IC50)	1000	nM	OE19 cells	Esophageal adenocarcinoma	CVCL_1622	[1]
Half Maximal Inhibitory Concentration (IC50)	1000	nM	SK-BR-3 cells	Breast adenocarcinoma	CVCL_0033	[1]
Half Maximal Inhibitory Concentration (IC50)	20	nM	HL-60 cells	Adult acute myeloid leukemia	CVCL_0002	[1]
Half Maximal Inhibitory Concentration (IC50)	20	nM	MOLM-13 cells	Adult acute myeloid leukemia	CVCL_2119	[1]
Half Maximal Inhibitory Concentration (IC50)	40	nM	MM1.S cells	Plasma cell myeloma	CVCL_8792	[1]
Half Maximal Inhibitory Concentration (IC50)	5	nM	Toledo cells	Diffuse large B-cell lymphoma	CVCL_3611	[1]

Each Antibody-drug Conjugate Related to This Payload

Full Information of The Activity Data of The ADC(s) Related to This Payload

Milatuzumab-RSL3-NH2 [Investigative]

ADC Info

Revealed Based on the Cell Line Data

Click To Hide/Show 1 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	2.40 nM	High CD74 expression (CD74+++)
Method Description	The inhibitory activity of Milatuzumab-RSL3-NH2 against cancer cell growth was evaluated by MTT cytotoxicity assay.
In Vitro Model	Diffuse large B-cell lymphoma	Toledo cells		CVCL_3611

Trastuzumab-RSL3-NH2 [Investigative]

ADC Info

Revealed Based on the Cell Line Data

Click To Hide/Show 1 Activity Data Related to This Level

Experiment 1 Reporting the Activity Date of This ADC					[1]
Efficacy Data	Half Maximal Inhibitory Concentration (IC50)	100.00 nM	High HER2 expression (HER2+++)
Method Description	The inhibitory activity of Trastuzumab-RSL3-NH2 against cancer cell growth was evaluated by MTT cytotoxicity assay.
In Vitro Model	Invasive breast carcinoma	BT-474 cells		CVCL_0179

References

Ref 1	The first ADC bearing the ferroptosis inducer RSL3 as a payload with conservation of the fragile electrophilic warhead. Eur J Med Chem. 2022 Dec 15;244:114863.