Payload Information
General Information of This Payload
| Payload ID | PAY0JNJHU |
|||||
|---|---|---|---|---|---|---|
| Name | FDA026-LE21 payload |
|||||
| Synonyms |
YSWPAY0017
Click to Show/Hide
|
|||||
| Structure |
|
|||||
| Formula | C30H33FN4O8S |
|||||
| Isosmiles | CC1=C(F)C=C2C3=C1CC[C@H](NC(CO)=O)C3=C(CN4C5=CC([C@](CC)(OCNCCS(C)(=O)=O)C6=O)=C(CO6)C4=O)C5=N2 |
|||||
| InChI |
InChI=1S/C30H33FN4O8S/c1-4-30(43-14-32-7-8-44(3,40)41)19-9-23-27-17(11-35(23)28(38)18(19)13-42-29(30)39)26-21(33-24(37)12-36)6-5-16-15(2)20(31)10-22(34-27)25(16)26/h9-10,21,32,36H,4-8,11-14H2,1-3H3,(H,33,37)/t21-,30-/m0/s1
|
|||||
| InChIKey |
YMIMHOQTRGIAKB-JRPXNJEYSA-N
|
|||||
| Pharmaceutical Properties | Molecule Weight |
628.679 |
Polar area |
165.92 |
||
Complexity |
1846.583405 |
xlogp Value |
1.23892 |
|||
Heavy Count |
44 |
Rot Bonds |
9 |
|||
Hbond acc |
11 |
Hbond Donor |
3 |
|||
Each Antibody-drug Conjugate Related to This Payload
Full Information of The Activity Data of The ADC(s) Related to This Payload
FDA026-LE21 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
46.3 nM
|
|||
| Method Description |
SK-BR-3 (ATCC) cells were selected as the cell line for in vitro activity detection experiments. 2000 cells were inoculated into each well of a 96-well cell culture plate and cultured for 20-24 hours. The antibody-drug conjugate prepared according to the method in Example 3 was formulated with L15 cell culture medium containing 10% FBS into 11 concentration gradients of test solution: 1000, 166.7, 55.6, 18.6, 6.17, 2.06, 0.69, 0.23, 0.08, 0.008 and OnM. 100 ul of the diluted test solution per well was added to the culture plate containing the inoculated cells and incubated in a 37°C, 5% CO, incubator for 144 hours. Then, CellTiter-Glo® Luminescent Cell Viability Assay Reagent (50 ul/well) was added, and the mixture was shaken at 500 rpm at room temperature for 10 minutes. The data were read using a SpectraMaxL microplate reader (OD570nm, with 2-second interval readings), and the IC50 results were calculated.
Click to Show/Hide
|
||||
| In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
89.34 nM
|
|||
| Method Description |
NCI-H820 (ATCC) cells were selected as the cell line for in vitro activity detection experiments. 2000 cells were inoculated into each well of a 96-well cell culture plate and cultured for 20-24 hours. The antibody-drug conjugate prepared according to the method in Example 3 was formulated with L15 cell culture medium containing 10% FBS into 11 concentration gradients of test solution: 1000, 166.7, 55.6, 18.6, 6.17, 2.06, 0.69, 0.23, 0.08, 0.008 and OnM. 100 ul of the diluted test solution per well was added to the culture plate containing the inoculated cells and incubated in a 37°C, 5% CO, incubator for 144 hours. Then, CellTiter-Glo® Luminescent Cell Viability Assay Reagent (50 ul/well) was added, and the mixture was shaken at 500 rpm at room temperature for 10 minutes. The data were read using a SpectraMaxL microplate reader (OD570nm, with 2-second interval readings), and the IC50 results were calculated.
Click to Show/Hide
|
||||
| In Vitro Model | Lung papillary adenocarcinoma | NCI-H820 cells | CVCL_1592 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) |
98.26 nM
|
|||
| Method Description |
22Rv1 (ATCC) cells were selected as the cell line for in vitro activity detection experiments. 2000 cells were inoculated into each well of a 96-well cell culture plate and cultured for 20-24 hours. The antibody-drug conjugate prepared according to the method in Example 3 was formulated with L15 cell culture medium containing 10% FBS into 11 concentration gradients of test solution: 1000, 166.7, 55.6, 18.6, 6.17, 2.06, 0.69, 0.23, 0.08, 0.008 and OnM. 100 ul of the diluted test solution per well was added to the culture plate containing the inoculated cells and incubated in a 37°C, 5% CO, incubator for 144 hours. Then, CellTiter-Glo® Luminescent Cell Viability Assay Reagent (50 ul/well) was added, and the mixture was shaken at 500 rpm at room temperature for 10 minutes. The data were read using a SpectraMaxL microplate reader (OD570nm, with 2-second interval readings), and the IC50 results were calculated.
Click to Show/Hide
|
||||
| In Vitro Model | Prostate carcinoma | 22RV1 cells | CVCL_1045 | ||