Linker Information
General Information of This Linker
| Linker ID |
LIN0XRXZL
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| Linker Name |
Dipeptide-diBrPD (dibromopyridazinedione )
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| Linker Type |
Flexible dual-reactive (thiol) linker; Cathepsin-cleavable linker
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| Antibody-Linker Relation |
Cleavable
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| Structure |
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| Formula |
C52H78Br2N12O15
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| Isosmiles |
CC(C)C(NC(=O)CCOCCOCCOCCn1nnc2c1CCC1C(CC2)C1COC(=O)NCCOCCOCCNC(=O)CCn1c(=O)c(Br)c(Br)c(=O)n1C)C(=O)NC(CCCNC(N)=O)C(=O)Nc1ccc(CO)cc1
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| InChI |
InChI=1S/C52H78Br2N12O15/c1-33(2)46(48(71)60-40(5-4-16-57-51(55)74)47(70)59-35-8-6-34(31-67)7-9-35)61-43(69)15-21-76-25-29-80-30-28-79-24-20-65-41-13-11-37-36(10-12-39(41)62-63-65)38(37)32-81-52(75)58-18-23-78-27-26-77-22-17-56-42(68)14-19-66-50(73)45(54)44(53)49(72)64(66)3/h6-9,33,36-38,40,46,67H,4-5,10-32H2,1-3H3,(H,56,68)(H,58,75)(H,59,70)(H,60,71)(H,61,69)(H3,55,57,74)
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| InChIKey |
NRTYWYSFLOAVLN-UHFFFAOYSA-N
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| Pharmaceutical Properties |
Molecule Weight
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1271.073
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Polar area
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350.94
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Complexity
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81
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xlogp Value
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1.0154
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Heavy Count
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81
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Rot Bonds
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37
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Hbond acc
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20
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Hbond Donor
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8
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Each Antibody-drug Conjugate Related to This Linker
Full Information of The Activity Data of The ADC(s) Related to This Linker
LRG1-ADC 5 [Investigative]
Discovered Using Cell Line-derived Xenograft Model
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Tumor Growth Inhibition value (TGI) | ≈ 52.40% (Day 16) | Positive LRG1 expression (LRG1+++/++) | ||
| Method Description |
Single-cell suspensions of 1 x106 B16F0 cells were injected subcutaneously into the lower back of Lrg1+/+ C57BL/6 mice in 100 mL PBS. Tumours were measured and therapy was initiated when tumour volumes reached 0.1 cm3. ADC was administered at a dose of 20 mg/kg treatments were administered by a single intraperitoneal injection every 7 days for 3 weeks.
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| In Vivo Model | Melanoma CDX model | ||||
| In Vitro Model | Melanoma | Melanoma cells | Homo sapiens | ||
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.90 nM
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Positive LRG1 expression (LRG1+++/++) | ||
| Method Description |
5 x104 cells were seeded in 96-well plates and incubated at 37 overnight. Cells were thenexposed to a range of concentrations of the test compounds diluted in growth medium at pH 6.5 and at 37 as ADC 5 (0100 nM, 72 h). MTT reagent (12 mM) was then added to each well and cells were incubated for 4 h at 37 , followed by the addition of DMSO and further incubation at 37 1C for 1 h.
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| In Vitro Model | Mouse melanoma | B16-F0 cells | CVCL_0604 | ||
References
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