Linker Information
General Information of This Linker
| Linker ID |
LIN0XJEDN
|
|||||
|---|---|---|---|---|---|---|
| Linker Name |
DBCO-PEG4-Gly (NH2)
|
|||||
| Linker Type |
Unclear
|
|||||
| Antibody-Linker Relation |
Cleavable
|
|||||
| Structure |
|
|||||
| Formula |
C31H40N4O7
|
|||||
| Isosmiles |
NCC(NCCOCCOCCOCCOCCC(NCCC(N1CC2=C(C#CC3=C1C=CC=C3)C=CC=C2)=O)=O)=O
|
|||||
| InChI |
InChI=1S/C31H40N4O7/c32-23-30(37)34-14-16-40-18-20-42-22-21-41-19-17-39-15-12-29(36)33-13-11-31(38)35-24-27-7-2-1-5-25(27)9-10-26-6-3-4-8-28(26)35/h1-8H,11-24,32H2,(H,33,36)(H,34,37)
|
|||||
| InChIKey |
ZAIXSOCHTUGYBQ-UHFFFAOYSA-N
|
|||||
| Pharmaceutical Properties |
Molecule Weight
|
580.682
|
Polar area
|
141.45
|
||
|
Complexity
|
1165.764745
|
xlogp Value
|
0.9707
|
|||
|
Heavy Count
|
42
|
Rot Bonds
|
19
|
|||
|
Hbond acc
|
8
|
Hbond Donor
|
3
|
|||
Each Antibody-drug Conjugate Related to This Linker
Full Information of The Activity Data of The ADC(s) Related to This Linker
ZA202500202A D04-F404-LP30 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
0.043 nM
|
Positive ROR1 expression (ROR1+++/++) | ||
| Method Description |
Ntera-2 cell at 625 cells/25 pL were seeded in a 384-well plat. ADCs were formulated at 2x starting concentration. Filter sterilized samples were serial diluted (1:3) under sterile conditions and added onto cells in triplicates. For cell viability measurement, 30 microliter of Cell Titer-Glo@ reagent (Promega Corp, Madison, WI) was added into each well, and plates processed as per product instructions.
Click to Show/Hide
|
||||
| In Vitro Model | Embryonal carcinoma | Ntera-2 cells | CVCL_0034 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) | > 10 nM | Negative ROR1 expression (ROR1-) | ||
| Method Description |
MCF-7 cells at 625 cells/25 pL were seeded in a 384-well plat. ADCs were formulated at 2x starting concentration. Filter sterilized samples were serial diluted (1:3) under sterile conditions and added onto cells in triplicates. For cell viability measurement, 30 microliter of Cell Titer-Glo@ reagent (Promega Corp, Madison, WI) was added into each well, and plates processed as per product instructions.
Click to Show/Hide
|
||||
| In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
References