Linker Information
General Information of This Linker
| Linker ID |
LIN0SPEIC
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| Linker Name |
P5 (PEG12)-VC-PAB
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| Linker Type |
Cathepsin-cleavable linker
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| Antibody-Linker Relation |
Cleavable
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| Structure |
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| Formula |
C51H83N6O19P
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| Isosmiles |
CC(C)[C@@H](C(N[C@H](C(NC1=CC=C(C=C1)CO)=O)CCCNC(N)=O)=O)NC(C2=CC=C(C=C2)NP(C#C)(OCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO)=O)=O
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| InChI |
InChI=1S/C51H83N6O19P/c1-4-77(64,76-39-38-75-37-36-74-35-34-73-33-32-72-31-30-71-29-28-70-27-26-69-25-24-68-23-22-67-21-20-66-19-18-65-17-16-58)57-45-13-9-43(10-14-45)48(60)56-47(41(2)3)50(62)55-46(6-5-15-53-51(52)63)49(61)54-44-11-7-42(40-59)8-12-44/h1,7-14,41,46-47,58-59H,5-6,15-40H2,2-3H3,(H,54,61)(H,55,62)(H,56,60)(H,57,64)(H3,52,53,63)/t46-,47-,77?/m0/s1
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| InChIKey |
MQYOGKQEJRQPSY-BEPASGIPSA-N
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| Pharmaceutical Properties |
Molecule Weight
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1115.222
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Polar area
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322.74
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Complexity
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1853.154516
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xlogp Value
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1.8925
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Heavy Count
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77
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Rot Bonds
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50
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Hbond acc
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19
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Hbond Donor
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8
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Each Antibody-drug Conjugate Related to This Linker
Full Information of The Activity Data of The ADC(s) Related to This Linker
37828728 ADC 3 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
15.5 ng/mL
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Positive HER2 expression (HER2+++/++) | ||
| Method Description |
To investigate the cytotoxicity of the unconjugated small-molecule TOP1 inhibitors and ADCs, respective cells were incubated for 4 days (small molecules) and 7 days (ADCs) with increasing concentrations of small molecules (0.015-1,000 nmol/L) and ADCs (0.05-3 ug/mL) to generate a dose-response curve. Killing was analyzed using resazurin cell viability dye at a final concentration of 55 umol/L (Merck) by dividing the fluorescence from control cells in medium by the fluorescence of ADC-treated cells. Fluorescence emission at 590 nmol/L was measured on a microplate reader Infinite 200 Pro (Tecan Group Ltd.).
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| In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
144.1 ng/mL
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Negative HER2 expression (HER2-) | ||
| Method Description |
To investigate the cytotoxicity of the unconjugated small-molecule TOP1 inhibitors and ADCs, respective cells were incubated for 4 days (small molecules) and 7 days (ADCs) with increasing concentrations of small molecules (0.015-1,000 nmol/L) and ADCs (0.05-3 ug/mL) to generate a dose-response curve. Killing was analyzed using resazurin cell viability dye at a final concentration of 55 umol/L (Merck) by dividing the fluorescence from control cells in medium by the fluorescence of ADC-treated cells. Fluorescence emission at 590 nmol/L was measured on a microplate reader Infinite 200 Pro (Tecan Group Ltd.).
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| In Vitro Model | Lung adenocarcinoma | HCC-78 cells | CVCL_2061 | ||
References