Linker Information
General Information of This Linker
| Linker ID |
LIN0KWZKA
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| Linker Name |
Tuneable thiol exchange linker 3
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| Linker Type |
Reduction sensitive linker
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| Antibody-Linker Relation |
Cleavable
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| Structure |
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| Formula |
C14H19N5O2S
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| Isosmiles |
OCC/S=C/C(C(C=C1)=CC=C1C(NCCN=[N+]=[N-])=O)=N/C
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| InChI |
InChI=1S/C14H19N5O2S/c1-16-13(10-22-9-8-20)11-2-4-12(5-3-11)14(21)17-6-7-18-19-15/h2-5,10,20,22H,6-9H2,1H3,(H,17,21)/b16-13-
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| InChIKey |
JPUVIQRZUCYXQN-SSZFMOIBSA-N
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| Pharmaceutical Properties |
Molecule Weight
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321.406
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Polar area
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110.45
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Complexity
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560.5608032
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xlogp Value
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1.4056
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Heavy Count
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22
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Rot Bonds
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8
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Hbond acc
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4
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Hbond Donor
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3
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Each Antibody-drug Conjugate Related to This Linker
Full Information of The Activity Data of The ADC(s) Related to This Linker
38888299 ADC 3 [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal inhibitory Concentration (lC50) | > 25 nM | Positive HER2 expression (HER2+++/++) | ||
| Method Description |
To investigate the cytotoxicity of the unconjugated small-molecule TOP1 inhibitors and ADCs, respective cells were incubated for 4 days (small molecules) and 7 days (ADCs) with increasing concentrations of small molecules (0.015-1,000 nmol/L) and ADCs (0.05-3 ug/mL) to generate a dose-response curve. Killing was analyzed using resazurin cell viability dye at a final concentration of 55 umol/L (Merck) by dividing the fluorescence from control cells in medium by the fluorescence of ADC-treated cells. Fluorescence emission at 590 nmol/L was measured on a microplate reader Infinite 200 Pro (Tecan Group Ltd.).
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| In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
References