We added 100 uL of media containing the drug to be tested (drug by concentration + 1 % FBS). We added PBS to surrounding wells to prevent evaporation. we cultivate in conditions of 37°C and 5 % CO2 according to the set time (Payload: initial observation and observes 48hr, 72hr, and 96hr culture conditions to select appropriate culture conditions). For Antibody and ADC, we selected appropriate culture conditions by observing culture conditions for 96hr, 120hr, and 144hr. In the case of 120hr and 144hr incubation conditions, we added additional media after 72hr incubation (The concentration of the drug is set to 3 below and 3 above based on the IC50 value used as a reference). Positive control usually uses etoposide 10 uM or 24 uM, and negative control uses DMSO with same concentration of drug and a positive control with SN38 or etoposide for validating the assay system (data not shown).

We added 100 uL of media containing the drug to be tested (drug by concentration + 1 % FBS). We added PBS to surrounding wells to prevent evaporation. we cultivate in conditions of 37°C and 5 % CO2 according to the set time (Payload: initial observation and observes 48hr, 72hr, and 96hr culture conditions to select appropriate culture conditions). For Antibody and ADC, we selected appropriate culture conditions by observing culture conditions for 96hr, 120hr, and 144hr. In the case of 120hr and 144hr incubation conditions, we added additional media after 72hr incubation (The concentration of the drug is set to 3 below and 3 above based on the IC50 value used as a reference). Positive control usually uses etoposide 10 uM or 24 uM, and negative control uses DMSO with same concentration of drug and a positive control with SN38 or etoposide for validating the assay system (data not shown).

We added 100 uL of media containing the drug to be tested (drug by concentration + 1 % FBS). We added PBS to surrounding wells to prevent evaporation. we cultivate in conditions of 37°C and 5 % CO2 according to the set time (Payload: initial observation and observes 48hr, 72hr, and 96hr culture conditions to select appropriate culture conditions). For Antibody and ADC, we selected appropriate culture conditions by observing culture conditions for 96hr, 120hr, and 144hr. In the case of 120hr and 144hr incubation conditions, we added additional media after 72hr incubation (The concentration of the drug is set to 3 below and 3 above based on the IC50 value used as a reference). Positive control usually uses etoposide 10 uM or 24 uM, and negative control uses DMSO with same concentration of drug and a positive control with SN38 or etoposide for validating the assay system (data not shown).

We added 100 uL of media containing the drug to be tested (drug by concentration + 1 % FBS). We added PBS to surrounding wells to prevent evaporation. we cultivate in conditions of 37°C and 5 % CO2 according to the set time (Payload: initial observation and observes 48hr, 72hr, and 96hr culture conditions to select appropriate culture conditions). For Antibody and ADC, we selected appropriate culture conditions by observing culture conditions for 96hr, 120hr, and 144hr. In the case of 120hr and 144hr incubation conditions, we added additional media after 72hr incubation (The concentration of the drug is set to 3 below and 3 above based on the IC50 value used as a reference). Positive control usually uses etoposide 10 uM or 24 uM, and negative control uses DMSO with same concentration of drug and a positive control with SN38 or etoposide for validating the assay system (data not shown).