HN02-L (5 mg/kg): BEL7402 (2×106) cells were injected subcutaneously into the right side of BALB/C nude mice to establish a hepatocellular carcinoma xenograft model. After the mean tumor volume reached 50-100 mm3, the tumor-bearing mice were divided randomly into 5 groups (eight mice/group). TG7 (5 mg/kg), HN02-L (5 mg/kg), HN02-H (10 mg/kg), or saline were injected intravenously every 3 days, and the positive group was treated with the drug sorafenib (20 mg/kg) by gavage daily for 3 w. Body weights and tumor volumes were measured periodically, and tumor volume was calculated using the formula V=LW2 /2 (L: long diameter of the tumor, W: short diameter of the tumor in the vertical direction). All mice were executed on day 21 of treatment, and tumors were excised for subsequent analysis.

HN02-H (10 mg/kg): BEL7402 (2×106) cells were injected subcutaneously into the right side of BALB/C nude mice to establish a hepatocellular carcinoma xenograft model. After the mean tumor volume reached 50-100 mm3, the tumor-bearing mice were divided randomly into 5 groups (eight mice/group). TG7 (5 mg/kg), HN02-L (5 mg/kg), HN02-H (10 mg/kg), or saline were injected intravenously every 3 days, and the positive group was treated with the drug sorafenib (20 mg/kg) by gavage daily for 3 w. Body weights and tumor volumes were measured periodically, and tumor volume was calculated using the formula V=LW2 /2 (L: long diameter of the tumor, W: short diameter of the tumor in the vertical direction). All mice were executed on day 21 of treatment, and tumors were excised for subsequent analysis.