Antibody Information
General Information of This Antibody
| Antibody ID | ANTI0DGSFU |
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| Antibody Name | LC4HC3 |
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| Organization | ADCENDO APS |
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| Synonyms |
LC4HC3
Click to Show/Hide
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| Antibody Type | Monoclonal antibody (mAb) |
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| Antigen Name | C-type mannose receptor 2 (MRC2) |
Antigen Info | ||||
| Click to Show/Hide the Sequence Information of This Antibody | ||||||
| Heavy Chain Sequence |
QVQLVQSGAEVKKPGASVKVSCKASGYIFIDYGMHWVRQAPGQRLEWMGSINTKSGVSTY
AAEFKGRVTIYSDTSASTAYMELSSLRSEDTAVYFCARPPYYSQYGSYWGQGTLVTVSSA STKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSG LYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGP SVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDEL TKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMHEALHNHYTQKSLSLSPGK Click to Show/Hide
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| Light Chain Sequence |
EIVMTQSPDSLAVSLGERATINCKASQNVDTYVVWYQQKPGQPPQPLIYSASSRFSGVPD
RFSGSGSGTDFTLTISSLQAEDVAIYYCQQYHNSPLTFGGGTKVEIKRTVAAPSVFIFPP SDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLT LSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC Click to Show/Hide
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The Activity Data of This Antibody
| Antibody Activity Information 1 | [1] | |||||
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Dissociation Constant (Kd)
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404000
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s-1
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U937 monocytic cells (IFN-Gamma stimulated) | CVCL_0007 | ||
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| Antigen Expression | Positive uPARAP expression (uPARAP+++/++) | |||||
| Antibody Function | LC4HC3 kinetic interaction analysis based on SPR measurements from U937 cells. | |||||
| Antibody Antigen Binding Assay | U937 cells ( 2x10<sup>3</sup> cells per well) were seeded at low density in a flatbottom 96 well plate in 90uL of medium and incubated overnight. The next day, LC4HC3 and LC0HC0 antibodies were preparenal as a serial dilution (1:4) in PBS and added in volumes of 10uL to each well, with a final maximum concentration of 0.1 ug/mL. Cells were incubated for 96 hours, before 12uL of CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS,Promega) was added, and incubated for an appropriate time for formation of color (around 60 minutes). The plates were then read at 490nm, with background subtraction at 630nm, using a plate reader, to yield the resulting viability estimates. Cells treated with PBS only were used as anontreated control, the viability of which the ADC treated wells were normalized to. | |||||
| Antibody Activity Information 2 | [1] | |||||
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Dissociation Constant (Kd)
|
404000
|
s-1
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U937 monocytic cells (IFN-Gamma stimulated) | CVCL_0007 | ||
| Antigen Expression | Positive uPARAP expression (uPARAP+++/++) | |||||
| Antibody Function | LC4HC3 kinetic interaction analysis based on SPR measurements from U937 cells. | |||||
| Antibody Antigen Binding Assay | U937 cells ( 2x10<sup>3</sup> cells per well) were seeded at low density in a flatbottom 96 well plate in 90uL of medium and incubated overnight. The next day, LC4HC3 and LC0HC0 antibodies were preparenal as a serial dilution (1:4) in PBS and added in volumes of 10uL to each well, with a final maximum concentration of 0.1 ug/mL. Cells were incubated for 96 hours, before 12uL of CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS,Promega) was added, and incubated for an appropriate time for formation of color (around 60 minutes). The plates were then read at 490nm, with background subtraction at 630nm, using a plate reader, to yield the resulting viability estimates. Cells treated with PBS only were used as anontreated control, the viability of which the ADC treated wells were normalized to. | |||||
Each Antibody-drug Conjugate Related to This Antibody