Antibody Information
General Information of This Antibody
| Antibody ID | ANI0KMCVI |
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| Antibody Name | B10v5 225-M |
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| Antibody Type | Bispecific antibody (BsAb) |
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| Antibody Subtype | Humanized IgG1-kappa |
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| Antigen Name | Epidermal growth factor receptor (EGFR); Hepatocyte growth factor receptor (MET) |
Antigen Info | ||||
Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
B10v5 225-M-vc-MMAE [Investigative]
Revealed Based on the Cell Line Data
| Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Inhibition rate (50 nM) |
91.00%
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High EGFR expression (EGFR+++/++); Low MET expression (MET-) | ||
| Method Description |
Cells were plated in 96-well tissue culture plates (SigmaAldrich) 1 day before treatment, serum-starved, and treated with serially diluted antibodies (0-167 nM in starvation medium) for 1 h.
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| In Vitro Model | Skin squamous cell carcinoma | A431 cells | CVCL_0037 | ||
| Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
1.00 nM
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High EGFR expression (EGFR+++/++); Low MET expression (MET-) | ||
| Method Description |
Cells were plated in 96-well tissue culture plates (SigmaAldrich) 1 day before treatment, serum-starved, and treated with serially diluted antibodies (0-167 nM in starvation medium) for 1 h.
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| In Vitro Model | Skin squamous cell carcinoma | A431 cells | CVCL_0037 | ||
| Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | Half Maximal Effective Concentration (EC50) |
5.90 nM
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| Method Description |
Cells were plated in 96-well tissue culture plates (SigmaAldrich) 1 day before treatment, serum-starved, and treated with serially diluted antibodies (0-167 nM in starvation medium) for 1 h.
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| In Vitro Model | Normal | NHEK-iPSCs #1 cells | CVCL_A4HY | ||
| Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
| Efficacy Data | 80% Effective Dose (ED80) |
4.40 nM
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High EGFR expression (EGFR+++/++); Low MET expression (MET-) | ||
| Method Description |
Cells were plated in 96-well tissue culture plates (SigmaAldrich) 1 day before treatment, serum-starved, and treated with serially diluted antibodies (0-167 nM in starvation medium) for 1 h.
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| In Vitro Model | Skin squamous cell carcinoma | A431 cells | CVCL_0037 | ||
References
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