Antibody Information
General Information of This Antibody
Antibody ID | ANI0JORAV |
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Antibody Name | Anti-GD2 mAb ch14.18 |
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Synonyms |
MAb-14.18; MOAB Ch14.18; DINUTUXIMAB; DINUTUXIMAB BETA
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Antibody Type | Monoclonal antibody (mAb) |
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Antibody Subtype | Chimeric IgG1-kappa |
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Antigen Name | Ganglioside GD2 (GD2) |
Antigen Info | ||||
ChEMBI ID | ||||||
DrugBank ID | ||||||
Drug Central ID | ||||||
Click to Show/Hide the Sequence Information of This Antibody | ||||||
Heavy Chain Sequence |
EVQLLQSGPELEKPGASVMISCKASGSSFTGYNMNWVRQNIGKSLEWIGAIDPYYGGTSY
NQKFKGRATLTVDKSSSTAYMHLKSLTSEDSAVYYCVSGMEYWGQGTSVTVSSASTKGPS VFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSS VVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHTCPPCPAPELLGGPSVFLFP PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLSPGK Click to Show/Hide
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Heavy Chain Varible Domain |
EVQLLQSGPELEKPGASVMISCKASGSSFTGYNMNWVRQNIGKSLEWIGAIDPYYGGTSY
NQKFKGRATLTVDKSSSTAYMHLKSLTSEDSAVYYCVSGMEYWGQGTSVTVSS Click to Show/Hide
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Heavy Chain Constant Domain 1 |
ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSS
GLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRV Click to Show/Hide
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Heavy Chain Constant Domain 2 |
APELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTK
PREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAK Click to Show/Hide
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Heavy Chain Constant Domain 3 |
GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDS
DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK Click to Show/Hide
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Heavy Chain Hinge Region |
EPKSCDKTHTCPPCP
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Heavy Chain CDR 1 |
GSSFTGYN
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Heavy Chain CDR 2 |
IDPYYGGT
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Heavy Chain CDR 3 |
VSGMEY
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Light Chain Sequence |
EIVMTQSPATLSVSPGERATLSCRSSQSLVHRNGNTYLHWYLQKPGQSPKLLIHKVSNRF
SGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHVPPLTFGAGTKLELKRTVAAPS VFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYS LSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC Click to Show/Hide
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Light Chain Varible Domain |
EIVMTQSPATLSVSPGERATLSCRSSQSLVHRNGNTYLHWYLQKPGQSPKLLIHKVSNRF
SGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHVPPLTFGAGTKLELK Click to Show/Hide
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Light Chain Constant Domain |
RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQD
SKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC Click to Show/Hide
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Light Chain CDR 1 |
QSLVHRNGNTY
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Light Chain CDR 2 |
KVS
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Light Chain CDR 3 |
SQSTHVPPLT
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Each Antibody-drug Conjugate Related to This Antibody
Full Information of The Activity Data of The ADC(s) Related to This Antibody
Ch14.18-MMAF [Investigative]
Discovered Using Cell Line-derived Xenograft Model
Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Tumor Growth Inhibition value (TGI) | ≈ 58.74% (Day 44) | Positive GD2 expression (GD2+++/++) | ||
Method Description |
When tumors reached 50 mm3 volume. Three groups received intravenous injections of 100 ug (5 mg/kg) ch14.18-MMAE, ch14.18-MMAF, or naked antibody for five times with an interval of 4 days, and the control group was injected with PBS.
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In Vivo Model | EL-4 CDX model | ||||
In Vitro Model | Thymoma | EL4 cells | CVCL_0255 | ||
Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Tumor Growth Inhibition value (TGI) | ≈ 74.95% (Day 43) | Positive GD2 expression (GD2+++/++) | ||
Method Description |
When tumors reached 50 mm3 volume. Three groups received intravenous injections of 100 ug (5 mg/kg) ch14.18-MMAE, ch14.18-MMAF, or naked antibody for five times with an interval of 4 days, and the control group was injected with PBS.
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In Vivo Model | B78-D14 CDX model | ||||
In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens |
Revealed Based on the Cell Line Data
Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.05 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens | ||
Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.06 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Thymoma | EL4 cells | CVCL_0255 | ||
Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.81 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Neuroblastoma | IMR-32 cells | CVCL_0346 | ||
Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
1.27 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | COLO 38 cells | CVCL_3934 | ||
Experiment 5 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
1.84 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Glioblastoma | T98G cells | CVCL_0556 | ||
Experiment 6 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
3.30 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | U2OS cells | CVCL_0042 | ||
Experiment 7 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
3.70 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Pleural malignant mesothelioma | MS-1 [Human mesothelioma] cells | CVCL_E993 | ||
Experiment 8 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
6.80 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Invasive breast carcinoma | Hs 578T cells | CVCL_0332 | ||
Experiment 9 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
8.00 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Astrocytoma | 1321N1 cells | CVCL_0110 | ||
Experiment 10 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
18.00 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
Experiment 11 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 20.00 nM | Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | MG-63 cells | CVCL_0426 | ||
Experiment 12 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Bone marrow neuroblastoma | SH-SY5Y cells | CVCL_0019 | ||
Experiment 13 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Neuroblastoma | NGP-127 cells | CVCL_UF75 | ||
Experiment 14 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Glioblastoma | U-87MG cells | CVCL_0022 | ||
Experiment 15 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | HOS cells | CVCL_0312 | ||
Experiment 16 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
Experiment 17 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | A375 cells | CVCL_0132 | ||
Experiment 18 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Melanoma | B16 cells | CVCL_F936 | ||
Experiment 19 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Malignant neoplasms of the mouse mammary gland | M3 cells | CVCL_4Y25 | ||
Experiment 20 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.02 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens | ||
Experiment 21 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.02 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Thymoma | EL4 cells | CVCL_0255 | ||
Experiment 22 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.19 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Glioblastoma | T98G cells | CVCL_0556 | ||
Experiment 23 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.25 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Neuroblastoma | IMR-32 cells | CVCL_0346 | ||
Experiment 24 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.30 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | U2OS cells | CVCL_0042 | ||
Experiment 25 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.30 nM
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High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | COLO 38 cells | CVCL_3934 | ||
Experiment 26 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.30 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Pleural malignant mesothelioma | MS-1 [Human mesothelioma] cells | CVCL_E993 | ||
Experiment 27 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.48 nM
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Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Invasive breast carcinoma | Hs 578T cells | CVCL_0332 | ||
Experiment 28 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
1.00 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Astrocytoma | 1321N1 cells | CVCL_0110 | ||
Experiment 29 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
5.30 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Bone marrow neuroblastoma | SH-SY5Y cells | CVCL_0019 | ||
Experiment 30 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
5.30 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
Experiment 31 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
5.30 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Amelanotic melanoma | A375 cells | CVCL_0132 | ||
Experiment 32 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
7.30 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | MG-63 cells | CVCL_0426 | ||
Experiment 33 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
12.40 nM
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Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Glioblastoma | U-87MG cells | CVCL_0022 | ||
Experiment 34 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Neuroblastoma | NGP-127 cells | CVCL_UF75 | ||
Experiment 35 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Osteosarcoma | HOS cells | CVCL_0312 | ||
Experiment 36 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
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In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
Experiment 37 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Melanoma | B16 cells | CVCL_F936 | ||
Experiment 38 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Malignant neoplasms of the mouse mammary gland | M3 cells | CVCL_4Y25 |
Ch14.18-MMAE [Investigative]
Discovered Using Cell Line-derived Xenograft Model
Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Tumor Growth Inhibition value (TGI) | ≈ 63.68% (Day 43) | Positive GD2 expression (GD2+++/++) | ||
Method Description |
When tumors reached 50 mm3 volume. Three groups received intravenous injections of 100 ug (5 mg/kg) ch14.18-MMAE, ch14.18-MMAF, or naked antibody for five times with an interval of 4 days, and the control group was injected with PBS.
|
||||
In Vivo Model | B78-D14 CDX model | ||||
In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens |
Revealed Based on the Cell Line Data
Experiment 1 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.29 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Thymoma | EL4 cells | CVCL_0255 | ||
Experiment 2 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.30 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Neuroblastoma | IMR-32 cells | CVCL_0346 | ||
Experiment 3 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.40 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | COLO 38 cells | CVCL_3934 | ||
Experiment 4 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.44 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens | ||
Experiment 5 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.51 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Pleural malignant mesothelioma | MS-1 [Human mesothelioma] cells | CVCL_E993 | ||
Experiment 6 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.68 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Glioblastoma | T98G cells | CVCL_0556 | ||
Experiment 7 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.69 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | U2OS cells | CVCL_0042 | ||
Experiment 8 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
0.93 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Invasive breast carcinoma | Hs 578T cells | CVCL_0332 | ||
Experiment 9 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
2.48 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Bone marrow neuroblastoma | SH-SY5Y cells | CVCL_0019 | ||
Experiment 10 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
4.28 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Astrocytoma | 1321N1 cells | CVCL_0110 | ||
Experiment 11 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
7.80 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | A375 cells | CVCL_0132 | ||
Experiment 12 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
9.40 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
Experiment 13 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) |
13.10 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | MG-63 cells | CVCL_0426 | ||
Experiment 14 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Neuroblastoma | NGP-127 cells | CVCL_UF75 | ||
Experiment 15 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Glioblastoma | U-87MG cells | CVCL_0022 | ||
Experiment 16 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | HOS cells | CVCL_0312 | ||
Experiment 17 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
Experiment 18 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Melanoma | B16 cells | CVCL_F936 | ||
Experiment 19 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | Half Maximal Inhibitory Concentration (IC50) | > 40.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Malignant neoplasms of the mouse mammary gland | M3 cells | CVCL_4Y25 | ||
Experiment 20 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.09 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Thymoma | EL4 cells | CVCL_0255 | ||
Experiment 21 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.14 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | COLO 38 cells | CVCL_3934 | ||
Experiment 22 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.15 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Neuroblastoma | IMR-32 cells | CVCL_0346 | ||
Experiment 23 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.20 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Pleural malignant mesothelioma | MS-1 [Human mesothelioma] cells | CVCL_E993 | ||
Experiment 24 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.22 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | B78-D14 cells | Homo sapiens | ||
Experiment 25 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.23 nM
|
High GD2 expression (GD2+++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Glioblastoma | T98G cells | CVCL_0556 | ||
Experiment 26 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.28 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | U2OS cells | CVCL_0042 | ||
Experiment 27 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.38 nM
|
Moderate GD2 expression (GD2++) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Invasive breast carcinoma | Hs 578T cells | CVCL_0332 | ||
Experiment 28 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.48 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Astrocytoma | 1321N1 cells | CVCL_0110 | ||
Experiment 29 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
0.51 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Bone marrow neuroblastoma | SH-SY5Y cells | CVCL_0019 | ||
Experiment 30 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
2.70 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Amelanotic melanoma | A375 cells | CVCL_0132 | ||
Experiment 31 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
2.98 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | MG-63 cells | CVCL_0426 | ||
Experiment 32 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
3.00 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Invasive breast carcinoma | MCF-7 cells | CVCL_0031 | ||
Experiment 33 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
8.47 nM
|
Low GD2 expression (GD2+) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Glioblastoma | U-87MG cells | CVCL_0022 | ||
Experiment 34 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) |
17.00 nM
|
Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Melanoma | B16 cells | CVCL_F936 | ||
Experiment 35 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Neuroblastoma | NGP-127 cells | CVCL_UF75 | ||
Experiment 36 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Osteosarcoma | HOS cells | CVCL_0312 | ||
Experiment 37 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Breast adenocarcinoma | SK-BR-3 cells | CVCL_0033 | ||
Experiment 38 Reporting the Activity Date of This ADC | [1] | ||||
Efficacy Data | 20% Maximal Inhibitory Concentration (IC20) | > 20.00 nM | Negative GD2 expression (GD2-) | ||
Method Description |
Cells were sub-cultured and seeded at 5,000 cells/well in complete growth medium in 96-well tissue culture plates, and incubated at 37°C, 5% CO2 overnight. Test reagents were serially diluted and added to the cell plates (initial concentration of 100 nM). Plates were incubated at 37°C, 5% CO2 for an additional 3 d.
|
||||
In Vitro Model | Malignant neoplasms of the mouse mammary gland | M3 cells | CVCL_4Y25 |
References
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